Polymerization of nucleotide-free, GDP- and GTP-bound cell division protein FtsZ: GDP makes the difference.
Sonia Huecas,José Manuel Andreu +1 more
TL;DR: Upon GTP hydrolysis by FtsZ polymers, in the absence of a continued GTP supply and under macromolecular crowding conditions enhancing FTSZ polymerization, the straight GTP polymers disappeared and were replaced by characteristic helically curved GDP‐bound polymers.
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About: This article is published in FEBS Letters. The article was published on 02 Jul 2004. and is currently open access. The article focuses on the topics: FtsZ & Tubulin.
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Citations
FtsZ in Bacterial Cytokinesis: Cytoskeleton and Force Generator All in One
TL;DR: Light microscope observations of how Z rings assemble in bacteria are reviewed, and evidence of how FtsZ generates a constriction force: by protofilament bending into a curved conformation is discussed.
661
Structural insights into the conformational variability of FtsZ.
TL;DR: New structures of FtsZ from Aquifex aeolicus, Bacillus subtilis, Methanococcus jannaschii and Pseudomonas aeruginosa are presented that provide strong constraints on any proposed role for a conformational switch in the Fts Z monomer and a new model in which lateral interactions help determine the curvature of protofilaments is proposed.
181
FtsZ protofilaments use a hinge-opening mechanism for constrictive force generation
Ying Li,Jen Hsin,Lingyun Zhao,Yiwen Cheng,Weina Shang,Kerwyn Casey Huang,Hong-Wei Wang,Sheng Ye,Sheng Ye +8 more
TL;DR: A complex and dynamic FtsZ protofilament network with a high degree of plasticity that is capable of generating forces to drive cytokinesis, during cycles of hydrolysis, while maintaining the structural integrity of individual monomers is suggested.
180
Structural mechanisms underlying nucleotide-dependent self-assembly of tubulin and its relatives.
Eva Nogales,Hong-Wei Wang +1 more
TL;DR: A two-state mechanism has been proposed for the recycling of alphabeta-tubulin during the microtubule disassembly-assembly cycle; this mechanism may be unique to eukaryotic dimeric tubulin and the micro Tubulin structure.
165
References
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
Thomas D. Pollard,Gary G. Borisy +1 more
TL;DR: A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematical models of the constituent reactions predict the rate of motion.
4K
How to measure and predict the molar absorption coefficient of a protein.
TL;DR: The studies reported here suggest that the Edelhoch method is the best method for measuring ε for a protein, which can best be predicted with this equation.
4K
Tubulin and FtsZ form a distinct family of GTPases.
TL;DR: The highly conserved nucleotide-binding sites of tubulin and FtsZ clearly differ from those of EF-Tu and other GTPases, while resembling the nucleotide site of glyceraldehyde-3-phosphate dehydrogenase, indicating a distinct family of GTP-hydrolyzing proteins.
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