Journal Article10.1016/0167-4889(86)90069-8
Platelet-activating factor as a stimulus of exocytosis in human neutrophils
Beatrice Dewald,Marco Baggiolini +1 more
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TL;DR: The results show that PAF-acether elicits marked secretion in untreated human neutrophils, and refute the prevalent view that cytochalasin B treatment is required for responsiveness.
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About: This article is published in Biochimica et Biophysica Acta. The article was published on 29 Aug 1986. The article focuses on the topics: Cytochalasin & Exocytosis.
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Citations
Formation and release of nitric oxide from human neutrophils and HL-60 cells induced by a chemotactic peptide, platelet activating factor and leukotriene B4
TL;DR: The data suggest that at least one NDRF is identical with NO or another labile NO containing compound derived from arginine, and the formation and release of NO was enhanced several‐fold in the presence of superoxide dismutase.
250
Chemiluminescence detection of H2O2 produced by human neutrophils during the respiratory burst.
TL;DR: The onset of fMLP-stimulated H2O2 generation was detectable by chemiluminescence within 2 s of stimulation (as opposed to more than double this time by fluorometry), showing that high sensitivity is an important consideration in evaluating respiratory burst kinetics.
153
Regulation of Capacitative Ca2+ Influx in Human Neutrophil Granulocytes ALTERATIONS IN CHRONIC GRANULOMATOUS DISEASE
TL;DR: It is shown that inhibition of the capacitative pathway is absent in various forms of chronic granulomatous disease and that upon physiological stimulation, Ca2+ entry into neutrophils is restricted by the depolarization accompanying O·̄2 production.
122
The onset of the respiratory burst in human neutrophils. Real-time studies of H2O2 formation reveal a rapid agonist-induced transduction process.
TL;DR: The results are consistent with a mechanism in which agonist signals appear to be transduced by two sequences acting in concert--a rate-limiting one liberating Ca2+ and diacylglycerol and turning on the Ca2-/phospholipid-dependent enzyme protein kinase C, and an essentially instantaneous one which does not appear to require further changes in cytosolic Ca2+.
118
Fatty‐acid‐induced activation of NADPH oxidase in plasma membranes of human neutrophils depends on neutrophil cytosol and is potentiated by stable guanine nucleotides
Roland Seifert,Günter Schultz +1 more
TL;DR: GTP[gamma S]), a potent activator of guanine-nucleotide-binding proteins (N-proteins), enhanced superoxide formation elicited by fatty acids up to fourfold, supporting the previous suggestion that NADPH oxidase is regulated by an N-protein.
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TL;DR: The chemokinetic and aggregating activities released from rat and human PMNs exposed to ionophore A23187 are not due to the release of mono-HETEs but to that of 5,12-di- HETE (leukotriene B), which is active over the concentration range 10 pg ml−1 to 5 ng ml-1.
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TL;DR: These short chain acylglyceryl ether phosphoglycerides represent an entirely new, potent and unique class of lipid chemical mediators.
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Biochemical and morphological characterization of azurophil and specific granules of human neutrophilic polymorphonuclear leukocytes
U. Bretz,Marco Baggiolini +1 more
TL;DR: Postnuclear supernates from homogenates of purified neutrophil polymorphonuclear leukocytes from human blood were fractionated by zonal sedimentation and isopycnic equilibration in sucrose gradients and identified a membrane-fraction characterized by the presence of the thiol-sensitive acid 4-nitrophenyl phosphatase and of alkaline phosphatase.
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