Journal Article10.1016/0378-1119(84)90136-7
Plasmid vectors for the selection of promoters
455
TL;DR: If a DNA fragment containing a promoter is inserted into one of the cloning sites upstream from the antibiotic genes, cells carrying such plasmids acquire resistance to cam or tet, and using these vectors two restriction fragments that contain promoters were identified.
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About: This article is published in Gene. The article was published on 01 Feb 1984. The article focuses on the topics: Plasmid & PBR322.
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Citations
Regulation of ribosomal RNA promoters with a synthetic lac operator
Jürgen Brosius,Adriana Holy +1 more
TL;DR: A synthetic 21-base-pair long DNA fragment containing the central lac operator sequence has been inserted near the initiation point of the cloned Escherichia coli rrnB rRNA promoter P2 in the natural and reverse orientation.
490
Expression of the RND-Type Efflux Pump AdeABC in Acinetobacter baumannii Is Regulated by the AdeRS Two-Component System
TL;DR: The AdeABC pump of Acinetobacter baumannii BM4454, which confers resistance to various antibiotic classes including aminoglycosides, is composed of the AdeA, AdeB, and AdeC proteins as mentioned in this paper.
403
ClpB is the Escherichia coli heat shock protein F84.1.
TL;DR: R reverse transcription of in vivo mRNA is used to show that the clpB gene was expressed from a sigma 32-specific promoter consensus sequence at both 37 and 42 degrees C and caused the appearance of a second protein spot, F68.5, on two-dimensional gels of heat-induced E. coli proteins.
364
Molecular characterization and protein analysis of the cap region, which is essential for encapsulation in Bacillus anthracis.
TL;DR: It was found that the capsule of B. anthracis conferred strong resistance to phagocytosis upon the bacterial host, and these three cistrons appeared to be membrane-associated enzymes mediating the polymerization of D-glutamic acid via the membrane.
349
Plasmid vector pBR322 and its special-purpose derivatives--a review.
Paulina Balbás,Xavier Soberón,Enrique Merino,Mario Zurita,Hilda Lomelí,Fernando Valle,Noemí Flores,Francisco Bolívar +7 more
TL;DR: A survey of derivative vectors based on the plasmid pBR322 to provide a survey of these derivative vectors and to summarize information currently available on pBR 322.
318
References
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
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Sequencing end-labeled DNA with base-specific chemical cleavages.
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TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
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Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
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A complementation analysis of the restriction and modification of DNA in Escherichia coli.
TL;DR: Intercistronic complementation was observed between three classes of restriction and modification mutants of E. coli B, indicating that at least three cistron (the ram cistrons) are involved in the genetic control of the [restriction and modification of DNA].
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