1. What have the authors contributed in "Ph-responsive benzaldehyde-functionalized peg-based polymeric nanoparticles for drug delivery: effect of preparation method on morphology, dye encapsulation and attachment" ?
After nanoparticle formation, the benzaldehyde groups on the surface are shown to react with an Alexa Fluor 488 hydroxylamine dye through oxime bond formation, illustra t in g the potential for these particles to be surface-functionalized with biologically important molecules, such as fluorescent dyes for tracking their intracellular fate or antibodies for targeted therapy.. Encapsulation of Nile Red and rhodamine 6G dyes is performed during the post-polymerization processing step for nanoparticle formation.. Nanoparticles with a fluorescent cargo were shown to be successfully internalized in both A2780 ovarian cancer and A549 lung epithelial human cells in vitro, further illustrating the potential for these formulations to be used as triggered release therapeutic drug delivery vehicles.
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2. What future works have the authors mentioned in the paper "Ph-responsive benzaldehyde-functionalized peg-based polymeric nanoparticles for drug delivery: effect of preparation method on morphology, dye encapsulation and attachment" ?
This added functionality opens up the possibility of actively targeting the nanoparticles to specific sites and/or cell types, in turn maximizing the efficacy of drug therapy whilst minimizing off target effects.. Alternatively, such active targeting has the potential to be of use in a diagnost ic setting, with, for instance, the nanoparticles accumulating at the desired site for imaging.. Using rhodamine 6G as a surrogate drug entity, the successful delivery of an encapsulated cargo was demonstrated, in vitro, in different cell lines, highlighting the potential for using these nanoformulations as drug delivery vehicles in a therapeutic setting.
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