Patent
PF rapid database construction method and application therefor
Jian Li,Zhen Heqiang,Caifen Zhang,Zhang Aiping,Xia Yingying,Chen Dayang,Xiandong Zhang,Saijun Liu,Li Wei,Huang Yile +9 more
- 31 Mar 2016
TL;DR: The PF rapid database construction method as mentioned in this paper comprises the following steps: a first step of fragmenting genomic DNA so as to obtain DNA fragments; a second step of performing end repairing on the DNA fragments, a third step of adding P1 and Barcode X adapters at the ends of the DNA fragment having undergone end repairing; a fourth step of library mixing according to the required yield of each library; a fifth step of nick translation on the mixed libraries; a sixth step of quality testing on the product having undergone nick translation.
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Abstract: A PF rapid database construction method and an application therefor. The PF rapid database construction method comprises the following steps: a first step of fragmenting genomic DNA so as to obtain DNA fragments; a second step of performing end repairing on the DNA fragments; a third step of adding P1 and Barcode X adapters at the ends of the DNA fragments having undergone end repairing; a fourth step of performing library mixing according to the required yield of each library; a fifth step of performing nick translation on the mixed libraries; a sixth step of performing quality testing on the product having undergone nick translation.
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References
Rapid Whole-Genome Sequencing for Genetic Disease Diagnosis in Neonatal Intensive Care Units
Carol J Saunders,Neil A. Miller,Neil A. Miller,Sarah E Soden,Sarah E Soden,Darrell L. Dinwiddie,Aaron Noll,Noor Abu Alnadi,Nevene Andraws,Melanie Patterson,Lisa Ann Krivohlavek,Joel Fellis,Sean Humphray,Peter Saffrey,Zoya Kingsbury,Jacqueline Weir,Jason Betley,Russell J. Grocock,Elliott H. Margulies,Emily G. Farrow,Michael Artman,Michael Artman,Nicole P. Safina,Nicole P. Safina,Joshua E. Petrikin,Kevin Hall,Stephen F. Kingsmore +26 more
TL;DR: A method that uses whole-genome sequencing (WGS) to achieve a differential diagnosis of genetic disorders in 50 hours rather than the 4 to 6 weeks is described and is intended to be a prototype for use in neonatal intensive care units.
Single Cell Genomics: Advances and Future Perspectives
Iain C. Macaulay,Thierry Voet +1 more
TL;DR: The major technological and biological breakthroughs achieved are reviewed, the remaining challenges to overcome are described, and a glimpse into the promise of recent and future developments are provided.
Patent
High-throughput sequencing joint applied to hybrid library building and library building method thereof
Jiang Zhi,Liu Shaoqing,Wu Jing,Peng Xianjun +3 more
- 20 Mar 2013
TL;DR: In this paper, a hybrid library building method which can be used for building multiple libraries is successfully built by combining a Truseq reagent library building and an NEB library building, so that the library building cost and time are saved and library building efficiency is improved.
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Multi-sample mixed sequencing method and kit
Daixing Zhou,Zhang Jianguang,Gao Yang,Wang Jun,Liu Qing +4 more
- 01 Apr 2015
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Establishment method of long mate pair library
Chen Zugeng,Nan Jiang,Ping Wang,Pu Yunjuan,Guiyou Liu,Chen Xiaoyun +5 more
- 09 Jan 2013
TL;DR: In this article, an establishment method of a long mate pair library was proposed, which consisted of the steps as follows: 1) randomly interrupting DNA (deoxyribose nucleic acid), and completing and repairing the gaps at the tail end; 2) preparing A-Fragment; 3) carrying out cohesive end connection to AFragment and LMP Adaptor; 4) digesting the LMP-Adaptor-Fragments with at least two restriction enzymes which can distinguish four basic groups; 5) cyclizing the fragments in the obtained enzyme digesting library again
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