Journal Article10.1021/LA001164W
Pepsin-gold colloid conjugates: preparation, characterization, and enzymatic activity
Anand Gole,Chandravanu Dash,Vidya Ramakrishnan,Sudhakar R. Sainkar,A.B. Mandale,Mala Rao,Murali Sastry +6 more
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TL;DR: The enzyme/colloidal gold conjugates were prepared by a simple protein-friendly process and the enzymatic activity of the bioconjugates is reported and the intactness of secondary and tertiary structures of the enzyme are reported.
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Abstract: Pepsin−colloidal gold conjugates were prepared by a simple protein-friendly process and the enzymatic activity of the bioconjugates is reported. The pepsin−gold conjugates are obtained by mixing colloidal gold and protein solutions at pH = 3 and, thereafter, centrifugation, washing, and redispersion of the pepsin−gold conjugate material in water. The bioconjugates in solution were characterized by UV−vis spectroscopy, fluorescence spectroscopy, and biocatalytic activity measurements while films of the bioconjugate material obtained by solvent evaporation on suitable substrates were further analyzed by scanning electron microscopy (SEM), energy dispersive analysis of X-rays (EDAX), transmission electron spectroscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). While TEM and SEM measurements showed aggregates of the enzyme/colloidal gold conjugates, the intactness of secondary and tertiary structures of the enzyme, as determined by FTIR and fluorescence s...
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References
Electron spin echo envelope modulation spectroscopic study of iron-nitrogen interactions in myoglobin hydroxide and Fe(III) tetraphenylporphyrin models.
TL;DR: The electron-nuclear coupling in low-spin iron complexes including myoglobin hydroxide (MbOH) and two related model compounds, Fe(III) tetraphenylporphyrin(pyridine)(OR-) (R = H or CH3) and Fe( III) tetrapsin(butylamine)(or-) was investigated using ESEEM spectroscopy.
34
Protein multilayer formation on colloids through a stepwise self-assembly technique.
Frank Caruso,Helmuth Möhwald +1 more
TL;DR: In this paper, multilayer films of the proteins fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) and immunoglobulin G (IgG) were assembled on 640 nm diameter polystyrene (PS) latex particles by their alternate deposition with either positively or negatively charged polyelectrolytes.
Concentration of proteins and removal of solutes.
TL;DR: The removal of low-molecular-weight solutes and concentration of protein solutions are the most frequently performed operations in protein purification and analysis.
Blueprint for a lipase support: Use of hydrophobic controlled-pore glasses as model systems.
John A. Bosley,John C. Clayton +1 more
TL;DR: Hydrophobic controlled‐pore glasses have been used as model systems for the immobilization of Rhizomucor miehei lipase and it has been found that pore diameters of at least 35 nm are needed for the lipase to be able to utilize the internal volume of the support particles in the immobilized process.
A xanthine oxidase/colloidal gold enzyme electrode for amperometric biosensor applications
TL;DR: In this article, an electrode has been prepared based on xanthine oxidase adsorbed to colloidal gold and evaporated onto the surface of glassy carbon, which is used to detect other substrates for xanthines such as 4-hydroxypyrimidine.