PCGF homologs, CBX proteins, and RYBP define functionally distinct PRC1 family complexes.

TL;DR: This protocol describes the production of GST-Cbx7 fusion proteins from E. coli by induced by isopropyl-beta-D-thiogalactopyranoside and they were purified by Glutathione Sepharose 4B.

TL;DR: This analysis demonstrated that Pax6, Tbr2, and Tbr1 form a direct feedforward genetic cascade, with direct feedback repression, that effect persistent changes of gene expression in cell lineages, to propagate features such as regional and laminar identity from progenitors to neurons.

TL;DR: This work identifies USP7 as a replisome-enriched SUMO deubiquitinase that is essential for DNA replication and provides a model explaining the differential accumulation of SUMO and Ub at replication forks and identifies an essential role ofUSP7 in DNA replication that should be considered in the development of USP6 inhibitors as anticancer agents.

TL;DR: The biochemistry and biology of methyllysine reader proteins are introduced, an overview of functions for those families of readers that have been targeted to date are provided, and the development of synthetic agents that directly block their methyllisine reading functions are reviewed.

TL;DR: The Genomic Regions Enrichment of Annotations Tool (GREAT) is developed to analyze the functional significance of cis-regulatory regions identified by localized measurements of DNA binding events across an entire genome.

TL;DR: The wild-type and mutant segmentation patterns are consistent with an antero-posterior gradient in repressor concentration along the embryo and a proximo-distal gradient along the chromosome in the affinities for repressor of each gene's cis-regulatory element.

TL;DR: This work has uncovered a role for non-coding RNA in the recruitment of PRC2 to target genes, and expanded the perspectives on its function and regulation.

TL;DR: It is shown that PcG proteins directly repress a large cohort of developmental regulators in murine ES cells, the expression of which would otherwise promote differentiation, and dynamic repression of developmental pathways by Polycomb complexes may be required for maintaining ES cell pluripotency and plasticity during embryonic development.