PARP-2, A Novel Mammalian DNA Damage-dependent Poly(ADP-ribose) Polymerase
Jean-Christophe Amé,V. Rolli,Valérie Schreiber,Claude Niedergang,Françoise Apiou,Patrice Decker,Sylviane Muller,Thomas Höger,Josiane Ménissier-de Murcia,Gilbert de Murcia +9 more
792
TL;DR: A cDNA encoding a 62-kDa protein that shares considerable homology with the catalytic domain of PARP-1 and also contains a basic DNA-binding domain is described, which is proposed to call this enzyme poly(ADP-ribose) polymerase 2 (PARP-2).
read more
About: This article is published in Journal of Biological Chemistry. The article was published on 18 Jun 1999. and is currently open access. The article focuses on the topics: Poly [ADP-Ribose] Polymerase 2 & Poly ADP ribose polymerase.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Identification of a novel GR-ARID1a-P53BP1 protein complex involved in DNA damage repair and cell cycle regulation
Felicity E. Stubbs,Benjamin P. Flynn,Caroline A Rivers,Matthew T. Birnie,Andrew Herman,Erin E. Swinstead,Songjoon Baek,Hai Fang,Jillian Temple,Jason S. Carroll,Gordon L. Hager,Stafford L. Lightman,Becky L. Conway-Campbell +12 more
TL;DR: In this article , the impact of siRNA knockdown of ARID1a compared to a functional interference approach in the HeLa human cervical cancer cell line was investigated, and it was shown that ARID 1a knockdown resulted in a significant global decrease in chromatin accessibility in ATAC-Seq analysis, as well as affecting a subset of genome-wide GR binding sites determined by analyzing GR ChIP-seq data.
Design, synthesis, biological evaluation of novel piperidine-based derivatives as potent poly(ADP-ribose) polymerase-1 (PARP-1) inhibitors
Chao Lin,Chang Liu,Zui Zou +2 more
- 01 May 2024
TL;DR: Researchers designed and synthesized novel piperidine-based PARP-1 inhibitors, demonstrating potent antiproliferative effects against breast cancer cells and inhibiting PARP-1 activity with IC50 values of 8.33-12.02 nM, suggesting potential as cancer therapeutics.
3
Patent
Nucleic acid molecules encoding a poly(adp-ribose) polymerase
Matthias Austen,Bernhard Luescher +1 more
- 11 Sep 2001
TL;DR: In this paper, nucleic acid molecules encoding a protein having poly(ADP-ribose) polymerase (PARP) activity as well as the encoded protein were described. And screening methods were described for compounds that act as agonist or antagonist of the protein having PARP activity.
2
Analysis of the DNA damage response in living cells
Oliver Mortusewicz
- 19 Dec 2007
TL;DR: Laser microirradiation and photobleaching techniques are employed in combination with specific mutants and inhibitors to analyze the real-time accumulation of proteins at laser-induced DNA damage sites in vivo, thus unravelling the mechanisms underlying the coordination of DNA repair in living cells.
2
References
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
254.4K
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
26.1K
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Andrew P. Feinberg
- 01 Jan 1984
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
22.6K
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs
TL;DR: 5'-Noncoding sequences have been compiled from 699 vertebrate mRNAs and GCCA/GCCATGG emerges as the consensus sequence for initiation of translation in vertebrates.
5.4K