PARP-2, A Novel Mammalian DNA Damage-dependent Poly(ADP-ribose) Polymerase

TL;DR: It is shown that PARP-1 and PARG control a cell death signal pathway that operates between five different cell compartments and communicates via three types of chemical messengers: a nucleotide, a cation, and proteins.

TL;DR: The results strongly suggest that the longevity-related differences in the poly(ADP-ribosyl)ation capacity of human lymphoblastoid cell lines cannot be explained by genetic polymorphisms in the PARP-1 coding sequence and that other mechanisms have to be considered as potential regulators of specific poly( ADP- ribosyl]ation capacity.

TL;DR: Cloning and characterization of a human cDNA encoding a novel nudix hydrolase NUDT5 for the hydrolysis of ADP-sugars and mNudT5, which has 81% amino acid identity to NUDt5 with catalytic activities similar to N UDT5 under the optimal pH of 9, are reported.

TL;DR: In this paper, the authors evaluated the impact of PARP-2 deletion on histopathological outcome from two different experimental models of cerebral ischemia and found that PARP deletion significantly increased neuronal cell loss in the hippocampal CA1 field (65%736% ischemic neurons) when compared with WT mice (31%733%).

TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.

TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.

TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.

TL;DR: 5'-Noncoding sequences have been compiled from 699 vertebrate mRNAs and GCCA/GCCATGG emerges as the consensus sequence for initiation of translation in vertebrates.