Parallelized wireless sensing system for continuous monitoring of microtissue spheroids
TL;DR: A wireless sensor scheme was developed, which subsequently has been used to detect the size variation of microtissues during long-term culturing and drug exposure assays, and offers great potential for automated electrical microtissue spheroid char-acterization.
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Abstract: Currently, the use of electrical readout methods for the investigation of microtissue spheroids in combination with lab automation tools is hindered by the cable connections that are required to interrogate the on-chip-integrated electrodes. To overcome this limitation, we developed a wireless sensor scheme, which can detect the size variation of microtissues during long-term culturing and drug exposure assays. The sensor system includes an interrogation board, which is composed of an inductor-capacitor (LC) readout circuit, and the tissue culture platform with integrated split-ring sensors. The magnetic coupling between the LC circuit and the sensors enables the interrogation of the on-chip sensors without any wire connection to the culture platform. By optimizing the sensor dimensions and the LC resonance frequencies, we were able to avoid cross talk between neighboring sensors. We integrated 12 tissue compartments on a standard microscopy slide with a sensor-to-sensor pitch of 9 mm, which is in accordance with standard 96-well plate dimensions. As a proof-of-concept experiment for the developed system, we monitored continuously and during more than four days the growth inhibition of colon cancer microtissue spheroids that had been exposed to different concentrations of doxorubicin, a chemotherapeutic compound. The stability of the measurements during long-term culturing and the compatibility of the sensor scheme with standard lab equipment offer great potential for automated electrical microtissue spheroid characterization.
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References
Spheroid-based drug screen: considerations and practical approach
TL;DR: This work describes a standardized setup for reproducible, easy-handling culture, treatment and routine analysis of multicellular spheroids, the classical 3D culture system resembling many aspects of the pathophysiological situation in human tumor tissue and provides a list of human carcinoma cell lines that produce treatable sp Heroids under identical culture conditions.
1.6K
Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning.
TL;DR: Common approaches to 3D culture are reviewed, the significance of 3D cultures in drug resistance and drug repositioning is discussed and some of the challenges of applying 3D cell cultures to high-throughput drug discovery are addressed.
3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained
Michele Zanoni,Filippo Piccinini,Chiara Arienti,Alice Zamagni,Spartaco Santi,R. Polico,Alessandro Bevilacqua,Anna Tesei +7 more
TL;DR: The analyses, carried out using a novel open source software capable of performing an automatic image analysis of 3D tumor colonies, showed that a number of morphology parameters affect the response of large spheroidids to treatment and indicate the need for a pre-selection of tumor spheroids of homogeneous volume and shape before use in a cytotoxicity test.
Graphene-based wireless bacteria detection on tooth enamel
Manu Sebastian Mannoor,Hu Tao,Jefferson D. Clayton,Amartya Sengupta,David L. Kaplan,Rajesh R. Naik,Naveen Verma,Fiorenzo G. Omenetto,Michael C. McAlpine +8 more
TL;DR: Graphene can be printed onto water-soluble silk, which permits intimate biotransfer of graphene nanosensors onto biomaterials, including tooth enamel, which is a fully biointerfaced sensing platform, which can be tuned to detect target analytes.
Single-cell microfluidic impedance cytometry: a review
Tao Sun,Hywel Morgan +1 more
TL;DR: The underlying principles of impedance analysis of particles are described and the state-of-the-art in the field of microfluidic impedance flow cytometry is described.
594