Parallel Evolution and Horizontal Gene Transfer of the pst Operon in Firmicutes from Oligotrophic Environments
Alejandra Moreno-Letelier,Alejandra Moreno-Letelier,Gabriela Olmedo,Luis E. Eguiarte,León P. Martínez-Castilla,Valeria Souza +5 more
TL;DR: The pst operon of Firmicutes shows two highly divergent clades with no correlation to the type of habitat nor a phylogenetic congruence, suggesting horizontal gene transfer.
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Abstract: The high affinity phosphate transport system (pst) is crucial for phosphate uptake in oligotrophic environments. Cuatro Cienegas Basin (CCB) has extremely low P levels and its endemic Bacillus are closely related to oligotrophic marine Firmicutes. Thus, we expected the pst operon of CCB to share the same evolutionary history and protein similarity to marine Firmicutes. Orthologs of the pst operon were searched in 55 genomes of Firmicutes and 13 outgroups. Phylogenetic reconstructions were performed for the pst operon and 14 concatenated housekeeping genes using maximum likelihood methods. Conserved domains and 3D structures of the phosphate-binding protein (PstS) were also analyzed. The pst operon of Firmicutes shows two highly divergent clades with no correlation to the type of habitat nor a phylogenetic congruence, suggesting horizontal gene transfer. Despite sequence divergence, the PstS protein had a similar 3D structure, which could be due to parallel evolution after horizontal gene transfer events.
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Citations
Physiological Roles of the Dual Phosphate Transporter Systems in Low and High Phosphate Conditions and in Capsule Maintenance of Streptococcus pneumoniae D39.
TL;DR: A model in which pneumococcus maintains high Pi transport in high and low Pi conditions that is required for optimal capsule biosynthesis is supported.
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Eneas Aguirre-von-Wobeser,Gloria Soberón-Chávez,Luis E. Eguiarte,Gabriel Yaxal Ponce-Soto,Mirna Vázquez-Rosas-Landa,Valeria Souza +5 more
TL;DR: This work shows that strains of Pseudomonas from the water column at Cuatro Ciénegas have the potential to interact antagonistically with many closely related strains and that these interactions are usually not reciprocal.
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Travel, sex, and food: what's speciation got to do with it?
Valeria Souza,Luis E. Eguiarte,Michael Travisano,James J. Elser,Christine Rooks,Janet L. Siefert +5 more
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Comparative genomics of free-living Gammaproteobacteria: pathogenesis-related genes or interaction-related genes?
TL;DR: Comparing the genomes of eight bacteria from human‐impacted environments with those of free‐living bacteria from the Cuatro Ciénegas Basin, a relatively pristine oligotrophic site, found that the CCB strains had several virulence‐related genes, which leads to the idea that these genes are not necessarily involved in pathogenesis, but in interactions within microbial communities.
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