Journal Article10.1016/J.CBPC.2011.07.009
Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis.
Sebastián E. Sabatini,Iara Rocchetta,Iara Rocchetta,Daniel E. Nahabedian,Daniel E. Nahabedian,Carlos Marcelo Luquet,María R. Eppis,Laura Bianchi,María del Carmen Ríos de Molina,María del Carmen Ríos de Molina +9 more
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TL;DR: The presence of intrACEllular rhodanine-positive granules, suggests copper accumulation in intracellular vacuoles of digestive cells in filter-feeding freshwater mussel Diplodon chilensis.
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Abstract: The aim of this work was to study the oxidative stress effects and histological alterations caused by dietary copper on the filter-feeding freshwater mussel Diplodon chilensis. Bivalves were fed during 6 weeks with the green algae Scenedesmus vacuolatus previously exposed to copper. Metal concentration in algae cultures and bivalve digestive gland was measured by TXRF. A maximum accumulation of 0.49 μg Cu/mg protein was detected at week 6. Also at this week, the hepatosomatic index (HSI) showed the highest decrease (50%) in response to Cu exposure. SOD and GST activities were significantly increased at weeks 4, 5 and 6, reaching an activity on average 50% higher than in controls for GST. CAT activity and GSH increased significantly at weeks 5 and 6. Despite this response, oxidative damage measured as TBARS and carbonyl groups contents increased significantly at weeks 4, 5 and 6, respectively. Digestive tubule and duct atrophy and cell-type replacement in treated mussels were observed by histological studies. The presence of intracellular rhodanine-positive granules, suggests copper accumulation in intracellular vacuoles of digestive cells.
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Citations
Modulating effects of orally supplied Euglena gracilis on the physiological responses of the freshwater mussel Diplodon chilensis, exposed to sewage water pollution in a Patagonian river (Argentina).
Virginia Angélica Bianchi,Juan Manuel Castro,Iara Rocchetta,Visitación Conforti,Mariano Martin Pascual,Carlos Marcelo Luquet +5 more
TL;DR: Results show that the total hemocyte number decreases while pollution load increases along the river course for both, EG and SV mussels, and diet and transplantation procedures may produce negative effects on the oxidative balance of gills and digestive gland and should be taken into account for bioremediation strategies.
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Assessment of oxidative stress, genotoxicity and histopathological responses in the digestive gland of Ruditapes decussatus collected from northern Tunisian lagoons
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TL;DR: The evident changes of antioxidant defence systems and macromolecules between the studied lagoons reveal the perturbation of the physiological states of clams from polluted sites thatope with seasonal changes and trace element accumulations.
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