Osmolyte effects on protein stability and solubility: a balancing act between backbone and side-chains.
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TL;DR: 1:1 correspondence between predicted and measured m-values covering a range of 12 kcal/mol/M in protein stability for 46 proteins and 9 different osmolytes is shown.
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About: This article is published in Biophysical Chemistry. The article was published on 01 Nov 2011. and is currently open access. The article focuses on the topics: Osmolyte & Side chain.
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Citations
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References
Living with water stress: evolution of osmolyte systems
TL;DR: Osmolyte compatibility is proposed to result from the absence of osmolytes interactions with substrates and cofactors, and the nonperturbing or favorable effects of oSMolytes on macromolecular-solvent interactions.
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Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses
TL;DR: Organic osmolytes are small solutes used by cells of numerous water-stressed organisms and tissues to maintain cell volume and have applications in biotechnology, agriculture and medicine, including in vitro rescue of the misfolded protein of cystic fibrosis.
Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants.
Marcelo M. Santoro,D. W. Bolen +1 more
TL;DR: The independence of delta epsilon N-U on denaturant supports the linear extension of pre- and postdenaturational base lines into the transition zone, allowing evaluation of unfolding equilibrium constants based on the two-state assumption.
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