Journal Article10.1126/SCIENCE.AAD1886
Opposing intrinsic temporal gradients guide neural stem cell production of varied neuronal fates
Zhiyong Liu,Ching-Po Yang,Ken Sugino,Chi-Cheng Fu,Chi-Cheng Fu,Ling-Yu Liu,Xiaohao Yao,Luke P. Lee,Tzumin Lee +8 more
TL;DR: This work searched for factors that could regulate neural temporal fate by RNA-sequencing lineage-specific neuroblasts at various developmental times and found that two RNA-binding proteins, IGF-II mRNA-binding protein (Imp) and Syncrip (Syp), display opposing high-to-low and low- to-high temporal gradients with lineage- specific temporal dynamics.
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Abstract: Neural stem cells show age-dependent developmental potentials, as evidenced by their production of distinct neuron types at different developmental times. Drosophila neuroblasts produce long, stereotyped lineages of neurons. We searched for factors that could regulate neural temporal fate by RNA-sequencing lineage-specific neuroblasts at various developmental times. We found that two RNA-binding proteins, IGF-II mRNA-binding protein (Imp) and Syncrip (Syp), display opposing high-to-low and low-to-high temporal gradients with lineage-specific temporal dynamics. Imp and Syp promote early and late fates, respectively, in both a slowly progressing and a rapidly changing lineage. Imp and Syp control neuronal fates in the mushroom body lineages by regulating the temporal transcription factor Chinmo translation. Together, the opposing Imp/Syp gradients encode stem cell age, specifying multiple cell fates within a lineage.
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Citations
Conservation and Divergence of Related Neuronal Lineages in the Drosophila Central Brain
Ying-Jou Lee,Ching-Po Yang,Yu-Fen Huang,Yisheng He,Qingzhong Ren,Hui-Min Chen,Rosa Linda Miyares,Hideo Otsuna,Yoshi Aso,Kei Ito,Tzumin Lee +10 more
TL;DR: This large-scale lineage mapping study reveals that relatively simple rules drive incredible neuronal complexity.
Regulation of developmental hierarchy in Drosophila neural stem cell tumors by COMPASS and Polycomb complexes
TL;DR: It is revealed that the trithorax/MLL1/2-COMPASS–like complex guides the developmental trajectory at the top of the tumor hierarchy, driving larval-to-embryonic temporal reversion and the marked expansion of CSCs that remain locked in a spectrum of early temporal states.
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A chromatin remodelling SWI/SNF subunit, Snr1, regulates neural stem cell determination and differentiation
TL;DR: In this paper , the Drosophila orthologue of SMARCB1, an ATP-dependent chromatin remodelling protein, identified a key role in regulating the transition of neuroepithelial cells into neural stem cells.
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Cell-type-specific interacting proteins collaborate to regulate the timing of Cyclin B protein expression in male meiotic prophase.
Catherine C. Baker,Lorenzo Gallicchio,Neuza Reis Matias,Douglas F Porter,Lucineh Cristina Parsanian,Emily Taing,Cheuk Ho Tam,Margaret T. Fuller +7 more
TL;DR: It is shown that the spermatocyte-specific protein Lut is required for translational repression of cycB in an 8-h window just before spermatocytes are fully mature, which choreograph the timing of expression of CycB protein during male meiotic prophase.
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Temporal evolution of single-cell transcriptomes of Drosophila olfactory projection neurons
Qijing Xie,Qijing Xie,Maria Brbic,Felix Horns,Sai Saroja Kolluru,Bob Jones,Jiefu Li,Jiefu Li,Anay R Reddy,Anay R Reddy,Anthony Xie,Sayeh Kohani,Zhuoran Li,Zhuoran Li,Colleen N McLaughlin,Tongchao Li,Chuanyun Xu,Chuanyun Xu,David Vacek,David J. Luginbuhl,Jure Leskovec,Stephen R. Quake,Liqun Luo,Hongjie Li +23 more
TL;DR: The single-cell transcriptomes of Drosophila olfactory projection neurons (PNs) at four developmental stages are obtained and it is discovered that PN transcriptomes reflect unique biological processes unfolding at each stage—neurite growth and pruning during metamorphosis at an early pupal stage; peaked transcriptomic diversity during Olfactory circuit assembly at mid-pupal stages; and neuronal signaling in adults.
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