Journal Article10.1126/SCIENCE.AAD1886
Opposing intrinsic temporal gradients guide neural stem cell production of varied neuronal fates
Zhiyong Liu,Ching-Po Yang,Ken Sugino,Chi-Cheng Fu,Chi-Cheng Fu,Ling-Yu Liu,Xiaohao Yao,Luke P. Lee,Tzumin Lee +8 more
TL;DR: This work searched for factors that could regulate neural temporal fate by RNA-sequencing lineage-specific neuroblasts at various developmental times and found that two RNA-binding proteins, IGF-II mRNA-binding protein (Imp) and Syncrip (Syp), display opposing high-to-low and low- to-high temporal gradients with lineage- specific temporal dynamics.
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Abstract: Neural stem cells show age-dependent developmental potentials, as evidenced by their production of distinct neuron types at different developmental times. Drosophila neuroblasts produce long, stereotyped lineages of neurons. We searched for factors that could regulate neural temporal fate by RNA-sequencing lineage-specific neuroblasts at various developmental times. We found that two RNA-binding proteins, IGF-II mRNA-binding protein (Imp) and Syncrip (Syp), display opposing high-to-low and low-to-high temporal gradients with lineage-specific temporal dynamics. Imp and Syp promote early and late fates, respectively, in both a slowly progressing and a rapidly changing lineage. Imp and Syp control neuronal fates in the mushroom body lineages by regulating the temporal transcription factor Chinmo translation. Together, the opposing Imp/Syp gradients encode stem cell age, specifying multiple cell fates within a lineage.
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Citations
A comprehensive temporal patterning gene network in Drosophila medulla neuroblasts revealed by single-cell RNA sequencing
TL;DR: In this paper, the authors performed single-cell RNA sequencing of Drosophila medulla neuroblasts of all ages to study the temporal patterning process with singlecell resolution.
Transcriptomic analysis of feminizing somatic stem cells in the Drosophila testis reveals putative downstream effectors of the transcription factor Chinmo.
TL;DR: This article showed that the transcription factor Chinmo is required to ensure the male sexual identity of somatic support cells in the Drosophila melanogaster testis and showed that loss of chinmo from male gonadal cells results in feminization: they transform from squamous to epithelial-like cells that resemble the female gonad but fail to properly ensheath the male germline.
Origin of wiring specificity in an olfactory map revealed by neuron type-specific, time-lapse imaging of dendrite targeting
TL;DR: In this paper , a two-photon and adaptive optical lattice light-sheet microscope-based time-lapse imaging reveals that projection neurons initiate active targeting with direction-dependent branch stabilization on the timescale of seconds.
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Transcriptional and epigenetic regulation of temporal patterning in neural progenitors.
TL;DR: Recently, the authors summarized recent advances in the study of temporal patterning of neural progenitors in Drosophila and vertebrates and discussed the involvement of epigenetic mechanisms, specifically the Polycomb group complexes and ATP-dependent chromatin remodeling complexes.
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scRNA-seq data from the larval Drosophila ventral cord provides a resource for studying motor systems function and development.
Rosario Vicidomini,Saumitra Dey Choudhury,Tae Hee Han,Dragan Maric,Thomas Brody,Mihaela Serpe +5 more
TL;DR: It is shown that the neural lineages that comprise the adult VNC are already defined, but quiescent, at the larval stage, and a glutamate receptor subunit required for basal neurotransmission and homeostasis at the larval neuromuscular junction is discovered.
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