On the Decrease in Lateral Mobility of Phospholipids by Sugars
TL;DR: This work studied the interactions between sugars and phospholipid membranes in giant unilamellar vesicles with the fluorescent lipid analog 3,3'-dioctadecyloxacarbocyanine perchlorate incorporated and found that sucrose decreased the lateral mobility in the fully rehydrated, liquid crystalline membrane more than other sugars, including trehalose.
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About: This article is published in Biophysical Journal. The article was published on 01 Mar 2007. and is currently open access. The article focuses on the topics: Trehalose & Membrane fluidity.
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Citations
LRRK2 Controls an EndoA Phosphorylation Cycle in Synaptic Endocytosis
Samer Matta,Kristof Van Kolen,Raquel Cruz Carvalho da Cunha,Geert van den Bogaart,Geert van den Bogaart,Wim Mandemakers,Katarzyna Miskiewicz,Pieter-Jan De Bock,Vanessa A. Morais,Sven Vilain,Dominik Haddad,Lore Delbroek,Jef Swerts,Lucía Chávez-Gutiérrez,Giovanni Esposito,Guy Daneels,Eric Karran,Matthew Holt,Matthew Holt,Kris Gevaert,Diederik Moechars,Bart De Strooper,Patrik Verstreken +22 more
TL;DR: A model is proposed in which LRRK2 kinase activity is part of an EndoA phosphorylation cycle that facilitates efficient vesicle formation at synapses, and this work uncovers a regulatory mechanism that indicates that reduced L RRK2 Kinase activity facilitates EndOA membrane association, while increased kinases activity inhibits membrane association.
360
Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy
Radek Macháň,Martin Hof +1 more
TL;DR: This work discusses several key questions of lateral mobility investigation in planar lipid membranes, namely the influence which membrane and aqueous phase composition, choice of a fluorescent tracer molecule, frictional coupling between the two membrane leaflets and between membrane and solid support has on the lateral mobility of lipids.
274
Synaptotagmin-1 may be a distance regulator acting upstream of SNARE nucleation.
Geert van den Bogaart,Shashi Thutupalli,Jelger H Risselada,Karsten Meyenberg,Matthew Holt,Dietmar Riedel,Ulf Diederichsen,Stephan Herminghaus,Helmut Grubmüller,Reinhard Jahn +9 more
TL;DR: It is shown that at low ionic strength SNARE-mediated membrane fusion becomes strictly dependent on both Ca2+ and synaptotagmin-1, and functions as a distance regulator that tethers the liposomes too far from the plasma membrane for SNARE nucleation in the absence of Ca2+, but while bringing theliposomes close enough for membrane fusion in the presence ofCa2+.
Production of Isolated Giant Unilamellar Vesicles under High Salt Concentrations
Hannah E Stein,Hannah E Stein,Susann Spindler,Susann Spindler,Navid Bonakdar,Chun Wang,Vahid Sandoghdar,Vahid Sandoghdar +7 more
TL;DR: An overview of the existing methods for GUV production is given and efforts on forming single, free floating vesicles up to several tens of μm in diameter and at high yield in various buffer solutions with physiological ionic strength and pH are presented.
The metabolism of soluble carbohydrates related to chilling injury in peach fruit exposed to cold stress
Ke Wang,Xingfeng Shao,Yifu Gong,Yong Zhu,Hongfei Wang,Xinglong Zhang,Dandan Yu,Fang Yu,Qiu Ziyun,Hao Lu +9 more
TL;DR: In this article, the authors investigated the changes in soluble carbohydrate content, transcript levels for genes encoding related enzymes, and the ascorbic acid regeneration cycle in fruit stored at 0°C and 5°C for 28 days.
142
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