Oligomeric properties and signal peptide binding by Escherichia coli Tat protein transport complexes

TL;DR: Despite low levels of sequence homology, the combined data indicate structural and functional conservation in the Tat systems of these three bacterial species.

TL;DR: Ize et al. as mentioned in this paper used gene expression profiling of Escherichia coli supported by phenotypic analysis to investigate how cells respond to a defect in the Tat pathway and found that approximately 50% of the genes that were differentially expressed in the tatC mutant are linked to the envelope defect, with the products of many of these genes involved in self defense or protection mechanisms, including the production of exopolysaccharide.

TL;DR: In this paper, the authors constructed 31 unique cysteine substitutions in the first 42 residues of TatB. Each of the substitutions results in a TatB protein that is competent to support Tatdependent protein translocation.

TL;DR: This review will summarise recent advances in understanding of Tat transport, focusing in particular on the roles played by Tat signal peptides in protein targeting and translocation.

TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.

TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.

TL;DR: The unifying feature of all proteins that are transported out of the cytoplasm of gram-negative bacteria by the general secretory pathway is the presence of a long stretch of predominantly hydrophobic amino acids, the signal sequence.

TL;DR: Red-edge effects were discovered for electron-transfer and proton-transfer reactions if they depended on the dynamics of the environment and stimulated the emergence and development of cryogenic energy-selective and single-molecular techniques that became valuable tools in their own right in chemistry and biophysics research.