Nucleocytoplasmic Transport: The Soluble Phase
Iain W. Mattaj,Ludwig Englmeier +1 more
1.2K
TL;DR: Directionality of either import or export depends on association between a substrate and its receptor on one side of the nuclear envelope and dissociation on the other, and the Ran GTPase is critical in generating this asymmetry.
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Abstract: Active transport between the nucleus and cytoplasm involves primarily three classes of macromolecules: substrates, adaptors, and receptors. Some transport substrates bind directly to an import or an export receptor while others require one or more adaptors to mediate formation of a receptor-substrate complex. Once assembled, these transport complexes are transferred in one direction across the nuclear envelope through aqueous channels that are part of the nuclear pore complexes (NPCs). Dissociation of the transport complex must then take place, and both adaptors and receptors must be recycled through the NPC to allow another round of transport to occur. Directionality of either import or export therefore depends on association between a substrate and its receptor on one side of the nuclear envelope and dissociation on the other. The Ran GTPase is critical in generating this asymmetry. Regulation of nucleocytoplasmic transport generally involves specific inhibition of the formation of a transport complex; however, more global forms of regulation also occur.
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TL;DR: The phenotypes of recessive lethal mutations in the oho31 gene that result in a massive decrease or loss of zygotic Pendulin expression are described, suggesting that Pendulin normally acts as a blood cell tumor suppressor and may be required for the normal transmission and function of proliferative signals in the cells.
Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation.
M Ruhl,M Himmelspach,G M Bahr,F Hammerschmid,H Jaksche,B Wolff,H Aschauer,G K Farrington,H Probst,D Bevec +9 more
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A novel receptor-mediated nuclear protein import pathway
Victoria W. Pollard,W. Matthew Michael,Sara Nakielny,Mikiko C. Siomi,Fan Wang,Gideon Dreyfuss +5 more
TL;DR: It is shown here that M9-mediated nuclear import occurs by a novel pathway that is independent of the well-characterized, importin-mediated classical NLS pathway, and it is demonstrated that there are at least two receptor- mediated nuclear protein import pathways.
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