Journal Article10.1007/BF00020015
Nuclear factors binding to the extensin promoter exhibit differential activity in carrot protoplasts and cells.
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TL;DR: An additional novel DNA-binding activity that is present in extracts prepared from protoplasts but not in extracts from unwounded cells, designated EBF (extensin-binding protein), binds to a DNA fragment which when deleted results in a 48% reduction in expression.
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Abstract: The expression of the cell wall protein extensin, a hydroxyproline-rich glycoprotein, is induced by several different stimuli, including wounding The process of protoplast preparation mimics the wounding effect and results in the induction of extensin Using transient expression in protoplasts we analyzed several deletions of the extensin promoter We identified an important transcriptional regulatory element located between the two TATA boxes that characterize the extensin promoter Other regulatory elements, located further upstream between −719 to −658, are necessary for maximum level of expression Employing electrophoretic mobility shift assays and methylation interference experiments, we demonstrate the interaction of nuclear factors with these upstream regulatory elements In addition to the previously identified factors EGBF-1 and EGBF-2, which are mainly present in unwounded cells, we identified an additional novel DNA-binding activity that is present in extracts prepared from protoplasts but not in extracts from unwounded cells This factor, designated EBF (extensin-binding protein), binds to a DNA fragment which when deleted results in a 48% reduction in expression
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Dynamic Aspects of the Plant Extracellular Matrix
TL;DR: This chapter discusses dynamic aspects of the plant extracellular matrix and describes the current status of knowledge of biochemical regulation that leads to differential patterns of synthesis of wall components, their site of synthesis and deposition, and the changes that occur in response to developmental and environmental cues.
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Matilde Jose,Pere Puigdomènech +1 more
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Complete elimination of maternal mitochondrial DNA during meiosis resulting in the paternal inheritance of the mitochondrial genome in Chlamydomonas species.
TL;DR: It is suggested that the complete elimination of maternal mtDNA during meiosis is the primary cause of paternal mitochondrial inheritance.
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Rhizobia modulate root-hair-specific expression of extensin genes.
TL;DR: Three cDNAs (ext3, ext127, and ext26), originally isolated by differential screening from a root-hair cDNA library of Vigna unguiculata, were found to encode extensin-like cell wall proteins, and the function of these homologous regions in gene regulation is unknown.
A gene for Brassica napus extensin is differentially expressed on wounding
TL;DR: Application of the wounding stimulus results in the accumulation of extensin gene transcripts to different degrees and at different times in the aerial parts of the plant, and results in a decline in the same transcripts in the roots.
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