NEBcutter: a program to cleave DNA with restriction enzymes
TL;DR: NEBcutter, version 1.0, is a program available via a web server that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence.
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Abstract: NEBcutter, version 1.0, is a program available via a web server (http://tools.neb.com/NEBcutter) that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence. It produces a variety of outputs including restriction enzyme maps, theoretical digests and links into the restriction enzyme database, REBASE (http://www.neb.com/rebase). Importantly, its table of recognition sites is updated daily from REBASE and it marks all sites that are potentially affected by DNA methylation (Dam, Dcm, etc.). Many options exist to choose the enzymes used for digestion, including all known specificities, subsets of those that are commercially available or sets of enzymes that produce compatible termini.
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Citations
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•Journal Article
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References
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
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TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
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TL;DR: The history and present situation of R-M, as well as other aspects of human evolution, are reviewed in detail.
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Structure and function of type II restriction endonucleases
Alfred Pingoud,Albert Jeltsch +1 more
TL;DR: Type II restriction endonucleases recognize short, usually palindromic, sequences of 4-8 bp and, in the presence of Mg(2+), cleave the DNA within or in close proximity to the recognition sequence.
Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.
TL;DR: A table of known site-specific DNA modification methyltransferases and their specificities is presented along with EMBL database accession numbers for cloned genes.