Book Chapter10.1002/9781118717769.CH4
Multiple Immunochemical Staining Techniques
Sofia Koch
- 30 Dec 2016
- pp 103-122
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About: The article was published on 30 Dec 2016. The article focuses on the topics: Confocal microscopy & Antigen retrieval.
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References
Controls for Immunocytochemistry: An Update
TL;DR: A new classification of immunocytochemical controls is proposed that will help in understanding this most important component of the experiment, where control samples are required to show label localization is correct.
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Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance.
TL;DR: The robust reliability of the simultaneous use of multiple mouse monoclonal antibodies of different immunoglobulin G (IgG) subclasses in a wide variety of multiplexing applications employing anti-mouse IgG subclass-specific secondary antibodies (2°Abs) is highlighted.
Multiple labeling techniques for fluorescence microscopy.
TL;DR: Although much information can be derived by localizing a single protein/peptide, it is often useful to label simultaneously for two or more antigens within the same cells or tissue sections.
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