Patent
Multilayered microfluidic DNA analysis system and method
Cynthia Ann Gorsuch Briscoe,Huinan Yu,Piotr Grodzinski,Robert Marrero,Jeremy W. Burdon,Rong-Fong Huang +5 more
- 10 Jan 2003
101
TL;DR: In this article, a multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together.
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Abstract: A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.
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