Journal Article10.1210/ENDO-121-3-1123
Monoclonal antibodies to human progesterone receptor: characterization by biochemical and immunohistochemical techniques.
Christine L. Clarke,Richard J. Zaino,Peter D. Feil,Jeri Miller,M. E. Steck,B. M. Ohlsson-Wilhelm,Pondichery G. Satyaswaroop +6 more
137
TL;DR: Seven monoclonal antibodies directed against human PR have been prepared, and their suitability for the study of PR by biochemical and immunohistochemical techniques has been demonstrated.
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Abstract: Progesterone receptor (PR) from a human endometrial carcinoma (EnCa 101) grown in nude mice consists of two hormone-binding proteins with mol wt around 116,000 and 85,000. To generate monoclonal antibodies against this receptor, PR was partially purified from EnCa 101 and used to immunize Robertsonian mice. Immune mouse spleens were fused with HL- 1 Friendly myeloma-653 cells, and hybridomas were screened by solid phase dot-blot assay and double antibody precipitation. Seven stable hybridomas were obtained, designated hPRa 1-7. Subisotyping revealed that hPRa 1 and 6 were immunoglobulin G2b, while the remainder were immunoglobulin Gx. Ultracentrifugation in high salt sucrose gradients showed that six of the seven antibodies effected a shift of [3H]progestin-labeled PR from EnCa 101; only hPRa 4 was ineffective in this regard. Protein blots of EnCa 101 cytosols and DEAE eluates revealed that hPRa 1, 3, 4, 5, and 7 recognized both PR proteins equally. hPRa 2 recognized principally the 116,000 mol wt PR prot...
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Citations
Physiological action of progesterone in target tissues.
TL;DR: Progesterone effects on proliferation and decidualization in the uterus during the menstrual cycle, and effects on lactation and Bone VIII.
1K
Steroid Hormone Receptors
E. V. Jensen
- 01 Jan 1991
TL;DR: The dependence of human cancers on hormones, the detection and characterization of steroid receptor interactions, and the physiological and pharmacological implications of steroid hormone receptors are discussed.
361
•Journal Article
Relative Expression of Progesterone Receptors A and B in Endometrioid Cancers of the Endometrium
Rebecca L. Arnett-Mansfield,Anna deFazio,Gerard Wain,Richard C. Jaworski,Karen Byth,Patricia A. Mote,Christine L. Clarke +6 more
TL;DR: Estimation of PRA and B expression by dual immunofluorescent histochemistry in endometrial adenocarcinomas compared with expression in normal and hyperplastic glands indicates that the decreased PR levels observed in these cancers arise from the loss of one PR isoform.
190
•Journal Article
Characterization of Progesterone Receptor A and B Expression in Human Breast Cancer
J D Graham,C Yeates,Rosemary L. Balleine,S S Harvey,J S Milliken,A M Bilous,Christine L. Clarke +6 more
TL;DR: In PR-positive breast tumors, the ratio of expression of PR-A and B proteins is close to unity, as is seen in a number of other progestin target tissues, but a significant proportion of tumors expressed very low levels ofPR-B and a consequently highPR-A/B ratio.
179
Colocalization of progesterone receptors in parvicellular dynorphin neurons of the ovine preoptic area and hypothalamus.
Chad D. Foradori,Lique M. Coolen,Maureen E. Fitzgerald,Donal C. Skinner,Robert L. Goodman,Michael N. Lehman +5 more
TL;DR: A dual-label immunoperoxidase procedure was used to visualize PRs and DYN in the preoptic area and hypothalamus of ovary-intact ewes killed during the luteal phase of the estrous cycle to test the hypothesis that an ultrashort feedback loop controls the release of DYN from ARC neurons.
153
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