Molecular tools in plant genetic resources conservation: a guide to the technologies.

TL;DR: Historical and contemporary gene flow within P. canariensis was studied with nuclear and chloroplast microsatellite markers, finding the high levels of gene flow detected would appear to have a positive effect on reforested stands by facilitating the immigration of local alleles from natural stands into potentially genetically depauperate first generation gene pools of reforesting stands.

TL;DR: It is inferred that botanical features such as predominantly selfing characteristics, long lifespan, and insects-pollinated trait lead to the survival of abundant genetic variation and special genetic structure in Chinese cherry.

TL;DR: The knowledge of existing plant genetic diversity is crucial for effective management of crop genetic resources and botanical classification, morphological, biochemical and molecular characterization are schemes used for measurement of genetic diversity.

TL;DR: DNA amplification parameters (Buffer composition, MgCl2, DNA template, primer, dNTPs, Taq DNA polymerase, sample volume, thermocycler program) were optimized to give reproducible bands.

TL;DR: The sensitivity of the commonly used progressive multiple sequence alignment method has been greatly improved and modifications are incorporated into a new program, CLUSTAL W, which is freely available.

TL;DR: The purpose of this discussion is to offer some unity to various estimation formulae and to point out that correlations of genes in structured populations, with which F-statistics are concerned, are expressed very conveniently with a set of parameters treated by Cockerham (1 969, 1973).

TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.