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Molecular Replacement Method
Patrick Argos,Michael G. Rossman +1 more
- 01 Jan 1972
416
TL;DR: The term molecular replacement encompasses a variety of techniques designed to utilize the occurrence of a molecule, molecular subunit, or molecular fragment in more than one crystallographic environment for the purpose of phase refinement.
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Abstract: The term molecular replacement encompasses a variety of techniques designed to utilize the occurrence of a molecule, molecular subunit, or molecular fragment in more than one crystallographic environment for the purpose of phase refinement. The limited size of nature’s building blocks and the necessity of their aggregation into molecules with closed point symmetries (Crick and Watson, 1956; Monod et al., 1965) provide the basis of the subject and its application to macromolecules. For example, a particular compound may be induced to crystallize in different forms, or a given molecule might be composed of several identical subunits.
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Citations
Phaser crystallographic software
Airlie J. McCoy,Ralf W. Grosse-Kunstleve,Paul D. Adams,Martyn Winn,Laurent C. Storoni,Randy J. Read +5 more
TL;DR: A description is given of Phaser-2.1: software for phasing macromolecular crystal structures by molecular replacement and single-wavelength anomalous dispersion phasing.
Atomic structure and chemistry of human serum albumin.
Xiao Min He,Daniel C. Carter +1 more
TL;DR: The three-dimensional structure of human serum albumin has been determined crystallographically to a resolution of 2.8 Å and should provide insight into future pharmacokinetic and genetically engineered therapeutic applications of serumalbumin.
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Likelihood-enhanced fast translation functions.
TL;DR: It is shown here how linear and quadratic Taylor-series expansions and least-squares approximations of the maximum-likelihood translation function lead to likelihood-enhanced translation functions, which can be calculated by FFT and which are more sensitive to the correct translation than the traditional correlation-coefficient fast translation function.
Solving structures of protein complexes by molecular replacement with Phaser.
TL;DR: Four case studies in using maximum-likelihood molecular replacement, as implemented in the program Phaser, to solve structures of protein complexes are described.
Crystal structure of the soluble human 55 kd TNF receptor-human TNF beta complex: implications for TNF receptor activation.
David Banner,Allan D'Arcy,Wolfgang Janes,Reiner L. Gentz,Hans-Joachim Schoenfeld,Clemens Broger,Hansruedi Loetscher,Werner Lesslauer +7 more
TL;DR: The X-ray crystal structure of the complex of the extracellular domain of the human 55 kd tumor necrosis factor (TNF) receptor with human TNF beta has been determined and is likely to be representative of the nerve growth factor (NGF)/TNF receptor family as a whole.
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References
On the Nature of Allosteric Transitions: A Plausible Model
TL;DR: "It is certain that all bodies whatsoever, though they have no sense, yet they have perception, and whether the body be alterant or alterec, evermore a perception precedeth operation; for else all bodies would be like one to another."
8.8K
Structure of Small Viruses
Francis Crick,James D. Watson +1 more
TL;DR: Watson and Crick proposed that plant viruses consistently took one of two shapes because the two chemical components of which they were made up, ribonucleic acid surrounded by a large number of identical protein subunits, were assembled according to a general plan that was determined by "symmetry elements".
742
Crystal structure of sickle-cell deoxyhemoglobin at 5 A resolution.
TL;DR: Double strands, similar to those found in the Hb S crystal, can be incorporated into a fiber model that is consistent with available information on the structure of deoxy HB S fibers in vivo.
346
A method of positioning a known molecule in an unknown crystal structure
R. A. Crowther,D. M. Blow +1 more
TL;DR: Uiklro et al. as discussed by the authors proposed a method to solve the problem of the lack of data for the purpose of data augmentation in the context of data collection and analysis.
285
The structure of the protein disk of tobacco mosaic virus to 5A resolution.
TL;DR: An electron density map of the TMV disk at 5 Å resolution has been obtained using isomorphous replacement and non-crystallographic symmetry and the polypeptide chain can be traced with little ambiguity.
186