Molecular Evolution of Hepatitis A Virus: a New Classification Based on the Complete VP1 Protein
Mauro Costa-Mattioli,Juan Cristina,Héctor Romero,Raoul Perez-Bercof,Didier Casane,Rodney Colina,Laura García,Ines Vega,Graciela Glikman,Victor Romanowsky,Alejandro A. Castello,Elisabeth Nicand,Michelle Gassin,Sylviane Billaudel,Virginie Ferré +14 more
TL;DR: The analysis of the complete VP1 gene allowed insight into the mode of evolution of HAV and revealed the emergence of a novel variant with a 15-amino-acid deletion located on the VP1 region where neutralization escape mutations were found, which could be the first antigenic variant of Hav so far identified.
read more
Abstract: Hepatitis A virus (HAV) is a positive-stranded RNA virus in the genus Hepatovirus in the family Picornaviridae So far, analysis of the genetic variability of HAV has been based on two discrete regions, the VP1/2A junction and the VP1 N terminus. In this report, we determined the nucleotide and deduced amino acid sequences of the complete VP1 gene of 81 strains from France, Kosovo, Mexico, Argentina, Chile, and Uruguay and compared them with the sequences of seven strains of HAV isolated elsewhere. Overall strain variation in the complete VP1 gene was found to be as high as 23.7% at the nucleotide level and 10.5% at the amino acid level. Different phylogenetic methods revealed that HAV sequences form five distinct and well-supported genetic lineages. Within these lineages, HAV sequences clustered by geographical origin only for European strains. The analysis of the complete VP1 gene allowed insight into the mode of evolution of HAV and revealed the emergence of a novel variant with a 15-amino-acid deletion located on the VP1 region where neutralization escape mutations were found. This could be the first antigenic variant of HAV so far identified.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Infection with hepatitis A, B, C, and delta viruses among patients with acute hepatitis in Mongolia†
Bira Tsatsralt-Od,Masaharu Takahashi,Kazunori Endo,Osorjin Buyankhuu,Oidov Baatarkhuu,Tsutomu Nishizawa,Hiroaki Okamoto +6 more
TL;DR: It is shown that de novo infections of HAV, HBV, HCV, and HDV are prevalent among young adults in Mongolia and that type D acute hepatitis is acquired due to superinfection of HDV on a background of chronic HBV infection.
52
Evidence of hepatitis A virus circulation in central Argentina: seroprevalence and environmental surveillance
Laura A. Yanez,Noelia S. Lucero,Patricia A. Barril,María del Pilar Díaz,María M. Tenaglia,Lorena Spinsanti,Silvia Nates,María Beatriz Isa,Viviana Ré +8 more
TL;DR: Environmental monitoring demonstrated that HAV circulates in the local population of Córdoba; therefore, health care systems should consider the implementation of preventive measures for susceptible adults in order to reduce the risk of HAV infection.
48
Hepatitis A virus evolution and the potential emergence of new variants escaping the presently available vaccines
Rosa M. Pintó,Lucía D’Andrea,Francisco J. Pérez-Rodriguez,M. Isabel Costafreda,Enric Ribes,Susana Guix,Albert Bosch +6 more
TL;DR: Some molecular and biological properties that allow Hepatitis A virus to live in a very quiescent way and to build an extremely stable capsid that is able to persist in and out of the body are reviewed.
Comparison between serum and saliva for the detection of hepatitis A virus RNA.
TL;DR: Oral fluid sample testing could be used as a noninvasive method of detecting HAV RNA during HAV outbreaks, and real-time PCR was more sensitive than nested RT-PCR.
45
Genetic relatedness among hepatitis A virus strains associated with food-borne outbreaks.
Gilberto Vaughan,Guo-liang Xia,Joseph C. Forbi,Michael A. Purdy,Livia Maria Gonçalves Rossi,Philip R. Spradling,Yury Khudyakov +6 more
TL;DR: Analysis of whole-genome sequences of 101 HAV strains identified from 4 major multi-state, food-borne outbreaks of hepatitis A in the Unites States and from 14 non-outbreak-related H AV strains that shared identical VP1/P2B sequences with the outbreak strains found no section of the HAV genome that could accurately represent phylogenetic relationships observed using WG sequences.
References
Clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
TL;DR: The sensitivity of the commonly used progressive multiple sequence alignment method has been greatly improved and modifications are incorporated into a new program, CLUSTAL W, which is freely available.
Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees.
Koichiro Tamura,Masatoshi Nei +1 more
TL;DR: In this paper, a new mathematical method for estimating the number of transitional and transversional substitutions per site, as well as the total number of nucleotide substitutions was proposed, taking into account excess transitions, unequal nucleotide frequencies, and variation of substitution rate among different sites.
Related Papers (5)
[...]