Molecular cloning of cDNA encoding the Ca2+ release channel (ryanodine receptor) of rabbit cardiac muscle sarcoplasmic reticulum.
Kinya Otsu,Huntington F. Willard,Vijay K. Khanna,Francesco Zorzato,N M Green,David H. MacLennan +5 more
635
TL;DR: Analysis of predicted secondary structures and hydropathy plots suggests that the two isoforms exhibit the same topology in both transmembrane and cytoplasmic domains, and suggests that a modulator binding domain in the protein lies between residues 2619 and 3016.
read more
About: This article is published in Journal of Biological Chemistry. The article was published on 15 Aug 1990. and is currently open access. The article focuses on the topics: Ryanodine receptor & Ryanodine receptor 2.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Caffeine-stimulated Ca2+ release from the intracellular stores of hepatocytes is not mediated by ryanodine receptors
Tracy J. McNulty,Colin W. Taylor +1 more
TL;DR: It is concluded that caffeine can specifically cause Ca2+ release from the intracellular stores of hepatocytes, but the effect is unlikely to be mediated by ryanodine receptors.
Structural analyses of human ryanodine receptor type 2 channels reveal the mechanisms for sudden cardiac death and treatment
Marco C. Miotto,Gunnar Weninger,Haikel Dridi,Qi Yuan,Yang Li,Anetta Wronska,Zephan Melville,Leah Sittenfeld,Steven Reiken,Andrew R. Marks +9 more
TL;DR: Together, these studies provide a mechanism for CPVT and for the therapeutic actions of ARM210, the Rycal drug ARM210 binds to RyR2-R2474S, reverting the primed state toward the closed state.
38
Modulation of ryanodine receptor Ca2+ channels (Review)
TL;DR: The modulation mechanisms of RyR proteins by associated proteins and phosphorylation are reviewed, finding that cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin-dependentprotein kinase II modulates the channel.
Modulation of the expression of ryanodine receptor mRNA from Plutella xylostella as a result of diamide insecticide application.
TL;DR: The relative expression levels of the RyRs from larvae were dependent on the insecticide concentration and gradually increased with increasing insecticide concentrations, while the weight of Px.RyR gradually decreased as the concentration of the diamides increased.
38
Luminal Ca2+ controls activation of the cardiac ryanodine receptor by ATP.
TL;DR: The results suggest that luminal Ca2+ ions potentiate the RYR2 gating activity in the presence of ATP predominantly by binding to a luminal site with an apparent affinity in the millimolar range, over which local luminalCa2+ likely varies in cardiac myocytes.
References
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
33.7K
A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
23.9K
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.