Molecular cloning and functional expression of cDNAs encoding a human Na+-nucleoside cotransporter (hCNT1)

TL;DR: This work has shown that like enzymes involved in drug metabolism, there are multiple and redundant transporters that may be responsible for the elimination of particular drugs.

TL;DR: This approach to positron emission tomography (PET) imaging may also complement imaging other aspects of tumor metabolism, such as energetics, protein and membrane synthesis, and apoptosis.

TL;DR: Development of molecular biology techniques has allowed identification of the proteins responsible for nucleoside transport processes, andPurification and N-terminal sequencing of the es transporter from human erythrocytes enabled cloning a human placental.

TL;DR: The isolation of a cDNA encoding a Na+-dependent nucleoside transporter from rat liver using an expression cloning strategy is reported, and the substrate specificities and kinetic characteristics of the cloned cotransporter are consistent with the properties of the Na-dependent, purine-selective nucleosid transporter in bile canalicular membranes.

TL;DR: A human gene (TBX2) is identified and cloned that exhibits strong sequence homology within a putative DNA binding domain to the drosophila optomotor-blind (omb) gene and lesser homology to theDNA binding domain of the murine brachyury or T gene.

TL;DR: A Na+ gradient-energized, concentrative nucleoside transport system in canalicular membrane vesicles (CMV) from rat liver is demonstrated by studying [3H]adenosine uptake using a rapid filtration technique.

TL;DR: A major metabolite of zidovudine (3'-azido-3'-deoxythymidine, AZT), which previously had not been observed in a variety of experimental animals, was identified in samples of plasma and urine from cynomolgus monkeys and a patient treated with AZT.