Journal Article10.1152/AJPCELL.1997.272.2.C707
Molecular cloning and functional expression of cDNAs encoding a human Na+-nucleoside cotransporter (hCNT1)
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TL;DR: In this article, the identification of a new human nucleoside transporter protein by molecular cloning and functional expression of its cDNA has been reported by using expression selection in Xenopus oocytes to iso...
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Abstract: We report identification of a new human nucleoside transporter protein by molecular cloning and functional expression of its cDNA. Previously, we used expression selection in Xenopus oocytes to iso...
read more
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Citations
Substituted cysteine accessibility method analysis of human concentrative nucleoside transporter hCNT3 reveals a novel discontinuous region of functional importance within the CNT family motif (G/A)XKX3NEFVA(Y/M/F).
Melissa D. Slugoski,Amy M. L. Ng,Sylvia Y.M. Yao,Colin C. Lin,Ras Mulinta,Carol E. Cass,Carol E. Cass,Stephen A. Baldwin,James D. Young +8 more
TL;DR: A novel membrane-associated topology for a region of the protein (TM 11A) that includes the highly conserved CNT family motif (G/A)XKX3NEFVA(Y/M/F) is suggested to suggest a new revised 15-TM CNT membrane architecture.
13
Adenosine transport: Recent advances in the molecular biology of nucleoside transporter proteins
Lori L. Jennings,Carol E. Cass,Mabel W.L. Ritzel,Sylvia Y.M. Yao,James D. Young,Mark Griffiths,Stephen A. Baldwin +6 more
TL;DR: The current state of knowledge of the molecular biology of the Molecular Biology of the ENT and CNT protein families is summarized, focusing on the role of these proteins (r/hENT1, r/hent2, r-hCNT1,r-CNT2) in the transport of adenosine.
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Hepatocyte nuclear factor-4α and bile acids regulate human concentrative nucleoside transporter-1 gene expression
TL;DR: The concentrative nucleoside transporter-1 (CNT1) is a member of the solute carrier 28 (SLC28) gene family and is expressed in the liver, intestine, and kidneys and expression levels of CNT1 may affect the pharmacokinetics of these drugs and the outcome of drug therapy.
Human concentrative nucleoside transporter 3 is a determinant of fludarabine transportability and cytotoxicity in human renal proximal tubule cell cultures
Adam N. Elwi,Adam N. Elwi,Vijaya L. Damaraju,Vijaya L. Damaraju,Michelle Kuzma,Michelle Kuzma,Stephen A. Baldwin,James D. Young,Michael B. Sawyer,Michael B. Sawyer,Carol E. Cass,Carol E. Cass +11 more
TL;DR: Variations in hCNT3 abundance in renal proximal tubules, and hence nucleoside reabsorption, may explain interpatient variability in fludarabine’s pharmacokinetics and toxicities.
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Mechanisms of transport of nucleosides and nucleoside analogues in choroid plexus
TL;DR: This review focuses on the mechanisms of transport of nucleosides and nucleoside analogues in the choroid plexus, and specific emphasis is placed on the molecular and functional characteristics of various nucleosid transporters.
12
References
Nucleoside transport in normal and neoplastic cells
TL;DR: A successful application of this therapeutic approach will require the analysis of the nucleoside transport phenotype of individual tumors in order to identify those patients that may benefit from such therapy, and the development of antibodies and cDNA probes for the various nucleosid transporters is currently underway in several laboratories.
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Primary Structure and Functional Expression of a cDNA Encoding the Bile Canalicular, Purine-specific Na-Nucleoside Cotransporter
TL;DR: The isolation of a cDNA encoding a Na+-dependent nucleoside transporter from rat liver using an expression cloning strategy is reported, and the substrate specificities and kinetic characteristics of the cloned cotransporter are consistent with the properties of the Na-dependent, purine-selective nucleosid transporter in bile canalicular membranes.
161
Cloning and mapping of a human gene (TBX2) sharing a highly conserved protein motif with the Drosophila omb gene.
TL;DR: A human gene (TBX2) is identified and cloned that exhibits strong sequence homology within a putative DNA binding domain to the drosophila optomotor-blind (omb) gene and lesser homology to theDNA binding domain of the murine brachyury or T gene.
77
A nucleoside transporter is functionally linked to ectonucleotidases in rat liver canalicular membrane.
TL;DR: A Na+ gradient-energized, concentrative nucleoside transport system in canalicular membrane vesicles (CMV) from rat liver is demonstrated by studying [3H]adenosine uptake using a rapid filtration technique.
74
•Journal Article
Isolation and characterization of an ether glucuronide of zidovudine, a major metabolite in monkeys and humans.
Steven S. Good,Cecilia S. Koble,R Crouch,Robert L. Johnson,Janet Litster Rideout,P de Miranda +5 more
TL;DR: A major metabolite of zidovudine (3'-azido-3'-deoxythymidine, AZT), which previously had not been observed in a variety of experimental animals, was identified in samples of plasma and urine from cynomolgus monkeys and a patient treated with AZT.
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