Journal Article10.1021/ACSSYNBIO.8B00258
Modular Pathway Engineering of Bacillus subtilis To Promote De Novo Biosynthesis of Menaquinone-7
70
TL;DR: The final resulting strain MK3-MEP123-Gly2-Δ dhbB with simultaneous overexpression of menA, dxs, dxr, yacM-yacN, glpD and deletion ofdhbB enabled the yield of MK-7 to 69.5 ± 2.8 mg/L upon 144 h fermentation in a 2 L baffled flask.
read more
Abstract: Menaquinone-7 (MK-7), a valuable vitamin K2, plays an important role in the prevention of osteoporosis and cardiovascular calcification. We chose B. subtilis 168 as the chassis for the modular metabolic engineering design to promote the biosynthesis of MK-7. The biosynthetic pathway of MK-7 was categorized into four modules, namely, the MK-7 pathway (Module I), the shikimate (SA) pathway (Module II), the methylerythritol phosphate (MEP) pathway (Module III), and the glycerol metabolism pathway (Module IV). Overexpression of menA (Module I) resulted in 6.6 ± 0.1 mg/L of MK-7 after 120 h fermentation, which was 2.1-fold that of the starting strain BS168NU (3.1 ± 0.2 mg/L). Overexpression of aroA, aroD, and aroE (Module II) had a negative effect on the synthesis of MK-7. Simultaneous overexpression of dxs, dxr, yacM, and yacN (Module III) enabled the yield of MK-7 to 12.0 ± 0.1 mg/L. Moreover, overexpression of glpD (Module IV) resulted in an increase of the yield of MK-7 to 13.7 ± 0.2 mg/L. Furthermore, del...
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Bottom-up synthetic biology approach for improving the efficiency of menaquinone-7 synthesis in Bacillus subtilis
Xiu-Yan Ding,Zhiming Zheng,Genhai Zhao,Liang Wang,Han Wang,Qiang Yang,Meng-Lin Zhang,Luyao Li,Peng Wang +8 more
TL;DR: In this article , the authors used a bottom-up synthetic biology approach for the synthetic redesign of central carbon and to improve the adaptability between material and energy metabolism in Menaquinone-7 (MK-7) synthesis pathways.
Engineering Bacillus subtilis Cells as Factories: Enzyme Secretion and Value-added Chemical Production
TL;DR: In this article, the authors outline basic concepts for B. subtilis cell factory engineering, and describe several examples of its applications in the production of proteins and high-value metabolites.
13
Optimisation of the fermentation media to enhance the production of the bioactive isomer of vitamin menaquinone-7
TL;DR: In this article , the authors investigated the effect of the media composition on the isomer profile obtained from fermentation and determined the optimum media combination to increase the concentration of the all-trans isomer and diminish the production of cis Menaquinone-7.
Bacillus subtilis as a host for natural product discovery and engineering of biosynthetic gene clusters.
11 Mar 2024
TL;DR: Heterologous expression of natural product biosynthetic gene clusters in Bacillus subtilis is a powerful tool for natural product discovery and engineering. It allows the production of large quantities of bioactive compounds, the generation of new analogues, and the identification and characterization of novel natural products.
8
Comparative transcriptome analysis reveals the underlying mechanism for over-accumulation of menaquinone-7 in Bacillus subtilis natto mutant
TL;DR: The results help get insights into the underlying metabolic mechanism of MK-7 production in B. subtilis natto, and provide more potential molecular targets to be engineered for further strain improvement.
7
References
A new mathematical model for relative quantification in real-time RT-PCR.
TL;DR: This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript and presents a new mathematical model that needs no calibration curve.
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
TL;DR: In this paper, complementary oligodeoxyribonucleotide (oligo) primers and the polymerase chain reaction are used to generate two DNA fragments having overlapping ends, and these fragments are combined in a subsequent 'fusion' reaction in which the overlapping ends anneal, allowing the 3' overlap of each strand to serve as a primer for the three' extension of the complementary strand.
7.9K
The shikimate pathway.
Klaus M. Herrmann,Lisa M. Weaver +1 more
- 01 Jun 1999
TL;DR: The shikimate pathway links metabolism of carbohydrates to biosynthesis of aromatic compounds, the precursor of the aromatic amino acids and many aromatic secondary metabolites, and is the sole target for the herbicide glyphosate.
1.3K
Developments in the use of Bacillus species for industrial production
TL;DR: Developments from the understanding of the complex protein translocation machinery of Gram-positive bacteria should allow the resolution of current secretion challenges and make Bacillus species preeminent hosts for heterologous protein production.
1.2K