Modeling Development and Disease with Organoids

TL;DR: In this paper, a review discusses new single-cell genomic technologies that complement singlecell RNA sequencing by providing additional readouts of cellular state beyond the transcriptome, and highlight regression models as a simple yet powerful approach to relate gene expression to other aspects of cellular states.

TL;DR: This work performs whole-genome sequencing of the SKBR3 breast cancer cell line and patient-derived tumor and normal organoids from two breast cancer patients and inferred SVs and large-scale allele-specific copy number variants (CNVs) using an ensemble of methods to highlight the need for long-read sequencing of cancer genomes for the precise analysis of their genetic instability.

TL;DR: The recent advances in breast cancer stem cell studies are reviewed in terms of available biomarkers, identification and isolation methods, molecular mechanisms, and methods for studying their cellular origin and lineage development.

TL;DR: As the authors gain a more detailed picture of the human immune system, more sophisticated models can be built to better reflect this complexity, both enabling the discovery of new immunological mechanisms and facilitating translation into the clinic.

TL;DR: The expression pattern of Lgr5 suggests that it marks stem cells in multiple adult tissues and cancers, suggesting that it represents the stem cell of the small intestine and colon.

TL;DR: A human pluripotent stem cell-derived three-dimensional organoid culture system that develops various discrete, although interdependent, brain regions that include a cerebral cortex containing progenitor populations that organize and produce mature cortical neuron subtypes is developed.

TL;DR: Human diploid epidermis epidermal cells have been successfully grown in serial culture and it is possible to isolate keratinocyte clones free of viable fibroblasts, and human diploids keratinocytes appear to have a finite culture lifetime.

TL;DR: A technology that can be used to study infected, inflammatory, or neoplastic tissues from the human gastrointestinal tract is developed that might have applications in regenerative biology through ex vivo expansion of the intestinal epithelia.