Modeling Development and Disease with Organoids
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TL;DR: 3D culture technology allow embryonic and adult mammalian stem cells to exhibit their remarkable self-organizing properties, and the resulting organoids reflect key structural and functional properties of organs such as kidney, lung, gut, brain and retina, and hold promise to predict drug response in a personalized fashion.
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About: This article is published in Cell. The article was published on 16 Jun 2016. and is currently open access. The article focuses on the topics: Regenerative medicine.
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Induced Pluripotent Stem Cells and Genome-Editing Tools in Determining Gene Function and Therapy for Inherited Retinal Disorders
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Human conjunctiva organoids to study ocular surface homeostasis and disease.
Marie Bannier-Hélaouët,Jeroen Korving,Ziliang Ma,Harry Begthel,Amir Giladi,Mart M. Lamers,Willine J. van de Wetering,Nobuyo Yawata,Makoto Yawata,Vanessa L S LaPointe,Mor M. Dickman,Rachel Kalmann,Saskia M Imhoff,J. van Es,Carmen López-Iglesias,Peter J. Peters,Bart L Haagmans,Wei Wu,Hans Clevers +18 more
TL;DR: Together, human conjunctiva organoid cultures enable the study of conjunctival (patho)-physiology and long-term expanding organoids and air-liquid interface representing mouse and human conjunctiva are described.
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Aurora kinase A regulates liver regeneration through macrophages polarization and Wnt/β-catenin signaling
TL;DR: In this paper, the effect and mechanism of Aurora kinase A (AURKA) on liver regeneration were studied using a 70% hepatectomy model using human liver organoids.
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Functional impact of a congenital stationary night blindness type 2 mutation depends on subunit composition of Cav1.4 Ca2+ channels.
TL;DR: It is concluded that the IT mutation stabilizes channel opening and alters ion selectivity of Cav1.4 in a manner that is strengthened by exclusion of exon 47 and inclusion of β2X13 and α2δ-4.
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References
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.
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TL;DR: The expression pattern of Lgr5 suggests that it marks stem cells in multiple adult tissues and cancers, suggesting that it represents the stem cell of the small intestine and colon.
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Cerebral organoids model human brain development and microcephaly
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Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.
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TL;DR: Human diploid epidermis epidermal cells have been successfully grown in serial culture and it is possible to isolate keratinocyte clones free of viable fibroblasts, and human diploids keratinocytes appear to have a finite culture lifetime.
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Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium.
Toshiro Sato,Daniel E. Stange,Marc Ferrante,Marc Ferrante,Robert G.J. Vries,Johan H. van Es,Stieneke van den Brink,Winan J. van Houdt,Apollo Pronk,Joost van Gorp,Peter D. Siersema,Hans Clevers +11 more
TL;DR: A technology that can be used to study infected, inflammatory, or neoplastic tissues from the human gastrointestinal tract is developed that might have applications in regenerative biology through ex vivo expansion of the intestinal epithelia.
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