Modeling Development and Disease with Organoids
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TL;DR: 3D culture technology allow embryonic and adult mammalian stem cells to exhibit their remarkable self-organizing properties, and the resulting organoids reflect key structural and functional properties of organs such as kidney, lung, gut, brain and retina, and hold promise to predict drug response in a personalized fashion.
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About: This article is published in Cell. The article was published on 16 Jun 2016. and is currently open access. The article focuses on the topics: Regenerative medicine.
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Citations
Bridging live-cell imaging and next-generation cancer treatment.
Maria Alieva,Amber K L Wezenaar,Ellen J. Wehrens,Anne C Rios +3 more
TL;DR: Recent applications of live-cell imaging for uncovering both tumour heterogeneity in treatment response and the mode of action of cancer-targeting drugs are reviewed and their implication for next-generation precision medicine is discussed.
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Extracellular Matrix Stiffness in Lung Health and Disease.
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Modeling Host-Virus Interactions in Viral Infectious Diseases Using Stem-Cell-Derived Systems and CRISPR/Cas9 Technology
TL;DR: Recent advances in the study of viral pathogenesis and host-virus crosstalk arising from the use of iPSC, organoid, and CRISPR/Cas9 technologies are discussed.
Toward the next generation of vascularized human neural organoids
TL;DR: Recent technological advances in culture strategies to generate vascularized NOs (vNOs), with a special focus on cerebral- and retinal-organoid models are summarized, to generate an ever more reliable NO model in vitro for deciphering the codes of human CNS development, disease progression, and translational application.
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Organoid culture system for patient-derived lung metastatic osteosarcoma.
Aina He,Aina He,Yujing Huang,Wanying Cheng,Deng Zhang,Weiwei He,Yueqing Bai,Chao Gu,Zhongping Ma,Zhenfang He,Guifan Si,Bing Chen,David T. Breault,Min Dong,Dongxi Xiang,Dongxi Xiang +15 more
TL;DR: A fully described formula was provided for establishing lung metastatic OS organoids (OSOs) that were able to be maintained and serially propagated for at least six months and can be further utilized for studying various aspects of OS biology for precision medicine.
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References
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Toshiro Sato,Robert G.J. Vries,Hugo J. Snippert,Marc van de Wetering,Nick Barker,Daniel E. Stange,Johan H. van Es,Arie Abo,Pekka Kujala,Peter J. Peters,Hans Clevers +10 more
TL;DR: It is concluded that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.
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Identification of stem cells in small intestine and colon by marker gene Lgr5
Nick Barker,Johan H. van Es,Jeroen Kuipers,Pekka Kujala,Maaike van den Born,Miranda Cozijnsen,Andrea Haegebarth,Jeroen Korving,Harry Begthel,Peter J. Peters,Hans Clevers +10 more
TL;DR: The expression pattern of Lgr5 suggests that it marks stem cells in multiple adult tissues and cancers, suggesting that it represents the stem cell of the small intestine and colon.
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Cerebral organoids model human brain development and microcephaly
Madeline A. Lancaster,Magdalena Renner,Carol Anne Martin,Daniel Wenzel,Louise S. Bicknell,Matthew E. Hurles,Tessa Homfray,Josef M. Penninger,Andrew P. Jackson,Juergen A. Knoblich +9 more
TL;DR: A human pluripotent stem cell-derived three-dimensional organoid culture system that develops various discrete, although interdependent, brain regions that include a cerebral cortex containing progenitor populations that organize and produce mature cortical neuron subtypes is developed.
Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.
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TL;DR: Human diploid epidermis epidermal cells have been successfully grown in serial culture and it is possible to isolate keratinocyte clones free of viable fibroblasts, and human diploids keratinocytes appear to have a finite culture lifetime.
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Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium.
Toshiro Sato,Daniel E. Stange,Marc Ferrante,Marc Ferrante,Robert G.J. Vries,Johan H. van Es,Stieneke van den Brink,Winan J. van Houdt,Apollo Pronk,Joost van Gorp,Peter D. Siersema,Hans Clevers +11 more
TL;DR: A technology that can be used to study infected, inflammatory, or neoplastic tissues from the human gastrointestinal tract is developed that might have applications in regenerative biology through ex vivo expansion of the intestinal epithelia.
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