Modeling Development and Disease with Organoids
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TL;DR: 3D culture technology allow embryonic and adult mammalian stem cells to exhibit their remarkable self-organizing properties, and the resulting organoids reflect key structural and functional properties of organs such as kidney, lung, gut, brain and retina, and hold promise to predict drug response in a personalized fashion.
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About: This article is published in Cell. The article was published on 16 Jun 2016. and is currently open access. The article focuses on the topics: Regenerative medicine.
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Citations
Carbon Nanotubes Promote the Development of Intestinal Organoids through Regulating Extracellular Matrix Viscoelasticity and Intracellular Energy Metabolism.
Lin Bao,Xuejing Cui,Xuejing Cui,Xiaoyu Wang,Junguang Wu,Mengyu Guo,Na Yan,Chunying Chen,Chunying Chen +8 more
TL;DR: In this article, the authors investigated the profitable effect of CNTs with a different graphene layer and surface modification on the 3D model of intestinal organoids and demonstrated that the promoted effect was highly dependent on the number of graphene layers.
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Smart Material Hydrogel Transfer Devices Fabricated with Stimuli-Responsive Silk-Elastin-Like Proteins.
Rachael N. Parker,Dana M. Cairns,Wenyao A. Wu,Kathryn E. Jordan,Chengchen Guo,Wenwen Huang,Zaira Martín-Moldes,David L. Kaplan +7 more
TL;DR: The design, characterization, and application of silk‐elastin‐like protein‐based smart carrier arrays for processing organoids show retention of native organoid features compared to controls without the hydrogel carrier system, thus avoiding any artifacts.
29
Generation and Culture of Human Pancreatic Ductal Adenocarcinoma Organoids from Resected Tumor Specimens.
Lindsey A. Baker,Lindsey A. Baker,Hervé Tiriac,Hervé Tiriac,David A. Tuveson,David A. Tuveson +5 more
TL;DR: Methods to generate PDA organoid cultures from resected human tumor specimens are described and how to propagate, cryopreserve, and thaw human PDAorganoid cultures are described.
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Controllable Fusion of Human Brain Organoids Using Acoustofluidics
TL;DR: A simple and versatile acoustofluidic method to improve the standardization of fused organoid models via a controllable spatial arrangement of organoids, which can promote neuron projection, projection maturation, and neural progenitor cell division in the assembloids.
3D fluid-dynamic ovarian cancer model resembling systemic drug administration for efficacy assay.
Alessandra Marrella,Gabriele Varani,Maurizio Aiello,Ivan Vaccari,Chiara Vitale,Martin Mojzisek,Cristina Degrassi,Silvia Scaglione +7 more
TL;DR: The results indicate that the in vivo-like dynamic environment provided by the MIVO® device allows to better model the 3D tumor environment and predict in vivo drug efficacy than a static in vitro model.
References
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.
Toshiro Sato,Robert G.J. Vries,Hugo J. Snippert,Marc van de Wetering,Nick Barker,Daniel E. Stange,Johan H. van Es,Arie Abo,Pekka Kujala,Peter J. Peters,Hans Clevers +10 more
TL;DR: It is concluded that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.
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TL;DR: The expression pattern of Lgr5 suggests that it marks stem cells in multiple adult tissues and cancers, suggesting that it represents the stem cell of the small intestine and colon.
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Cerebral organoids model human brain development and microcephaly
Madeline A. Lancaster,Magdalena Renner,Carol Anne Martin,Daniel Wenzel,Louise S. Bicknell,Matthew E. Hurles,Tessa Homfray,Josef M. Penninger,Andrew P. Jackson,Juergen A. Knoblich +9 more
TL;DR: A human pluripotent stem cell-derived three-dimensional organoid culture system that develops various discrete, although interdependent, brain regions that include a cerebral cortex containing progenitor populations that organize and produce mature cortical neuron subtypes is developed.
Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.
James G. Rheinwatd,Howard Green +1 more
TL;DR: Human diploid epidermis epidermal cells have been successfully grown in serial culture and it is possible to isolate keratinocyte clones free of viable fibroblasts, and human diploids keratinocytes appear to have a finite culture lifetime.
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Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium.
Toshiro Sato,Daniel E. Stange,Marc Ferrante,Marc Ferrante,Robert G.J. Vries,Johan H. van Es,Stieneke van den Brink,Winan J. van Houdt,Apollo Pronk,Joost van Gorp,Peter D. Siersema,Hans Clevers +11 more
TL;DR: A technology that can be used to study infected, inflammatory, or neoplastic tissues from the human gastrointestinal tract is developed that might have applications in regenerative biology through ex vivo expansion of the intestinal epithelia.
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