Microplate-test for the rapid determination of bacteriophage-susceptibility of Campylobacter isolates-development and validation.
TL;DR: A simple susceptibility test using 800 isolates of one Campylobacter strain with different degrees of susceptibility and four bacteriophages of the British phage typing scheme was developed and examined for its suitability and correlated well with the conventional method.
read more
Abstract: A simple susceptibility test using 800 isolates of one Campylobacter strain with different degrees of susceptibility and four bacteriophages of the British phage typing scheme was developed and examined for its suitability. The test presented is economically cheaper and less time consuming than the conventional agar overlay plate assay and therefore enables the monitoring of changes in the susceptibility pattern during phage therapy under practical field conditions. The main objective of this study was to compare the simplified test with the conventional agar overlay plate assay. The conventional test describes for a population of Campylobacter: i. the rate of resistant isolates (0 plaques) and ii. the degree of susceptibility, also called relative efficiency of plating (EOP), for the remaining isolates. The simplified test divides the isolates into four susceptibility ranks, which are easily distinguishable to the naked eye. Ten Campylobacter isolates out of each rank were subjected to the conventional method for validation of the simplified test. Each resistance rank contained isolates showing certain degrees of susceptibility, reflecting decreasing susceptibility by an increase of the rank. Thus, the simplified test correlated well with the conventional method. Nevertheless, it can be suggested for a clear cut to summarise the first thee ranks as "high susceptible" and to mark out the fourth rank as reduced susceptible. Further test improvements will enable the monitoring of the degree of susceptibility and potentially also of resistance during phage therapy in the field. To ensure a long-lasting successful use of phage therapy, further studies on both the loss of susceptibility and the development of resistance of Campylobacter against phages combined with their impact on phage therapy will be necessary.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Bacteriophage Procurement for Therapeutic Purposes.
Beata Weber-Dąbrowska,Ewa Jończyk-Matysiak,Maciej Żaczek,Małgorzata Łobocka,Marzanna Łusiak-Szelachowska,Andrzej Górski +5 more
TL;DR: This article attempts to summarize the state of the art: properties of phages, the methods for their isolation, criteria of phage selection for therapeutic purposes and limitations of their use.
Impact of a single phage and a phage cocktail application in broilers on reduction of Campylobacter jejuni and development of resistance.
TL;DR: The study demonstrated that the deployed phages persisted over the whole investigation period and the Campylobacter load was permanently reduced by the phage-cocktail as well as by the single phage.
Effect of Bacteriophage Application on Campylobacter jejuni Loads in Commercial Broiler Flocks
TL;DR: The results suggest that maximum reduction of Campylobacter at the slaughterhouse might be achieved by phage application 1 to 4 days prior to slaughter, which had proved to be effective in previous noncommercial studies under controlled experimental conditions.
Reduction of Campylobacter jejuni in Broiler Chicken by Successive Application of Group II and Group III Phages
Jens A. Hammerl,Claudia Jäckel,Thomas Alter,Pawel Janzcyk,Kerstin Stingl,Marie Theres Knüver,Stefan Hertwig +6 more
TL;DR: It is shown that group II and group III phages exhibit different kinetics of infection, which illustrated that Campylobacter phage cocktails have to be carefully composed to achieve the best results.
Campylobacter Phage Isolation and Characterization: What We Have Learned So Far
Claudia Jäckel,Jens A. Hammerl,Stefan Hertwig +2 more
- 15 Feb 2019
TL;DR: Specific issues that should be considered when Campylobacter phages are studied are addressed, starting with the isolation and propagation of the phages and ending with a thorough characterization including whole-genome sequencing.
24
References
Bacteriophage host range and bacterial resistance.
Paul Hyman,Stephen T. Abedon +1 more
TL;DR: How phage host range can (and should) be defined plus claims of host ranges spanning multiple bacterial genera are summarized plus bacterial mechanisms of phage resistance are reviewed.
745
Quantitative risk assessment of human campylobacteriosis associated with thermophilic Campylobacter species in chickens.
Hanne Rosenquist,Niels L. Nielsen,Helle Mølgaard Sommer,Birgit Nørrung,Bjarke Bak Christensen +4 more
TL;DR: Simulations designed to predict the effect of different mitigation strategies showed that the incidence of campylobacteriosis associated with consumption of chicken meals could be reduced 30 times by introducing a 2 log reduction of the number of Campylobacteria on the chicken carcasses.
549
Phage host range and efficiency of plating.
TL;DR: This chapter describes rapid procedures for determining the host range and relative EOP on each host of any phage.
470
Analysis of the baseline survey on the prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses in the EU, 2008, Part A: Campylobacter and Salmonella prevalence estimates
Efsa publication
- 01 Jan 2010
TL;DR: The results of the counts of Campylobacter on broiler carcasses showed substantial variation among the countries in contamination levels, andSalmonella Infantis and Salmonella Enteritidis were the two most frequently isolated serovars on broilers carcasses in the EU and accounted for about onethird and one-sixth of the Salmoneella isolates, respectively.
Bacteriophages: an appraisal of their role in the treatment of bacterial infections.
TL;DR: This review outlines those features of bacteriophages that contribute to their utility in therapy and explores the potential for their re-introduction into Western medicine.
416