Microbial-Derived Butyrate Promotes Epithelial Barrier Function through IL-10 Receptor-Dependent Repression of Claudin-2.
Leon Zheng,Caleb J. Kelly,Kayla D. Battista,Rachel E. M. Schaefer,Jordi M. Lanis,Erica E. Alexeev,Ruth Wang,Joseph C. Onyiah,Douglas J. Kominsky,Sean P. Colgan +9 more
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TL;DR: These findings provide a novel mechanism by which microbial-derived butyrate promotes barrier through IL-10RA–dependent repression of claudin-2 and enhance IEC barrier formation through activated Stat3 and HDAC inhibition.
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Abstract: Commensal interactions between the enteric microbiota and distal intestine play important roles in regulating human health. Short-chain fatty acids (SCFAs), such as butyrate, produced through anaerobic microbial metabolism represent a major energy source for the host colonic epithelium and enhance epithelial barrier function through unclear mechanisms. Separate studies revealed that the epithelial anti-inflammatory IL-10 receptor α subunit (IL-10RA) is also important for barrier formation. Based on these findings, we examined if SCFAs promote epithelial barrier through IL-10RA-dependent mechanisms. Using human intestinal epithelial cells (IECs), we discovered that SCFAs, particularly butyrate, enhanced IEC barrier formation, induced IL-10RA mRNA, IL-10RA protein, and transactivation through activated Stat3 and HDAC inhibition. Loss and gain of IL-10RA expression directly correlates with IEC barrier formation and butyrate represses permeability-promoting claudin-2 tight-junction protein expression through an IL-10RA-dependent mechanism. Our findings provide a novel mechanism by which microbial-derived butyrate promotes barrier through IL-10RA-dependent repression of claudin-2.
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