Journal Article10.1007/S002160100962
Microarray sampling-platform fabrication using bubble-jet technology for a biochip system.
85
TL;DR: A simple and cost-effective procedure for the fabrication of microarrays containing biological samples using a modified bubble-jet printing system is presented, illustrated by the detection of human fragile histidine triad (FHIT), a tumor suppressor gene.
read more
Abstract: The fabrication of microarrays containing PCR-amplified genomic DNA extracts from mice tumors on a Zetaprobe membrane using a modified thermal ink-jet printer is described. A simple and cost-effective procedure for the fabrication of microarrays containing biological samples using a modified bubble-jet printing system is presented. Because of their mass-produced design, ink-jet printers are a much cheaper alternative to conventional spotting techniques. The usefulness of the biochip microarray platform is illustrated by the detection of human fragile histidine triad (FHIT), a tumor suppressor gene. Subcutaneous carcinomas were induced with MKN/FHIT and MKN/E4 cell lines in immunodeficient mice. Several weeks into their development, the tumors from both groups of mice were removed and subjected to DNA extraction by lysis of tissue samples. The extracted DNA samples were amplified by PCR (30 cycles) using the primers corresponding to nucleotides 2 to 18 of the FHIT sequence. The resulting solution was transferred to the individual reservoirs of a three-color cartridge from a conventional thermal ink-jet printer (HP 694C), and arrays were printed on to a Zetaprobe membrane. After spotting, these membranes were used in a hybridization assay, using fluorescent probes, and detected with a biochip.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Inkjet printing for high-throughput cell patterning
TL;DR: The results indicate that commercial inkjet printing technology can be used to create viable cellular patterns with a resolution of 350 microm through the deposition of biologically active proteins.
764
Bio-microarray fabrication techniques--a review.
TL;DR: The current state of microarray fabrication is reviewed and technologies emerged from other applications and have the potential to increasemicroarray fabrication throughput; however, there are several challenges in applying them to micro array fabrication, including interference from satellite drops and biomolecule denaturization.
Inkjet printing for biosensor fabrication: Combining chemistry and technology for advanced manufacturing
TL;DR: The factors behind successful inkjet biosensor fabrication are reviewed, including printers, inks, patterning methods, and matrix types, and future possibilities for biosensors enabled by this novel combination of chemistry and technology are considered.
286
An amperometric glucose biosensor prototype fabricated by thermal inkjet printing.
Leonardo Setti,Alessandro Fraleoni-Morgera,Barbara Ballarin,A. Filippini,D. Frascaro,C. Piana +5 more
TL;DR: The reported findings indicate that inkjet printing could be a viable instrument for the easy construction of a working biosensor via direct digital design using biological and conductive polymer based inks.
246
3D Printing of Scaffold for Cells Delivery: Advances in Skin Tissue Engineering
TL;DR: The focus of this review is to describe the role of 3D architecture and arrangement of cells within this system that can heal wounds and aid in skin regeneration.
105