Microarray analysis provides new insights into the function of apolipoprotein O in HepG2 cell line
TL;DR: It is likely that apoO participates in fatty acid metabolism and the inflammatory response in HepG2 cells, and UCP2 may act as a mediator between lipid metabolism and inflammation in apOO-silenced HepG 2 cells.
read more
Abstract: Background: Apolipoprotein O (apoO) is a new member of the apolipoprotein family. However, data on its physiological functions are limited and inconsistent. Using a microarray expression analysis, this study explored the function of apoO in liver cells. Methods: HepG2 cells were treated either with oleic acid or tumor necrosis factor-α for 24 h. mRNA and protein expression of apoO were assessed by quantitative real-time PCR (qRT-PCR) and Western blot respectively. An efficient lentiviral siRNA vector targeting the human apoO gene was designed and constructed. The gene expression profile of HepG2 human hepatocellular carcinoma cells transfected with the apoO silencing vector was investigated using a whole-genome oligonucleotide microarray. The expression levels of some altered genes were validated using qRT-PCR. Results: ApoO expression in HepG2 cells was dramatically affected by lipid and inflammatory stimuli. A total of 282 differentially expressed genes in apoO-silenced HepG2 cells were identified by microarray analysis. These genes included those participating in fatty acid metabolism, such as ACSL4, RGS16, CROT and CYP4F11, and genes participating in the inflammatory response, such as NFKBIZ, TNFSF15, USP2, IL-17, CCL23, NOTCH2, APH-1B and N2N. The gene Uncoupling protein 2 (UCP2), which is involved in both these metabolic pathways, demonstrated significant changes in mRNA level after transfection. Conclusions: It is likely that apoO participates in fatty acid metabolism and the inflammatory response in HepG2 cells, and UCP2 may act as a mediator between lipid metabolism and inflammation in apoO-silenced HepG2 cells.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Genome-wide association analysis of egg production performance in chickens across the whole laying period
TL;DR: Findings in this study could provide promising genes and SNP markers to improve egg production performance based on marker-assisted breeding selection, while further functional validation is still needed in other populations.
Acyl-CoA synthase ACSL4: an essential target in ferroptosis and fatty acid metabolism.
TL;DR: In this article , the authors focus on the structure, biological function, and unique role of ACSL4 in various human diseases and propose a potential therapeutic target, which facilitates the cross-talk between ferroptosis and FA metabolism.
39
Regulators of G-protein-signaling proteins: negative modulators of G-protein-coupled receptor signaling.
TL;DR: Human and animal studies have revealed that RGS proteins play a vital role in physiology and can be ideal targets for diseases such as those related to addiction where receptor signaling seems continuously switched on.
32
PPARδ activation induces hepatic long-chain acyl-CoA synthetase 4 expression in vivo and in vitro
TL;DR: It is demonstrated that PPARδ activation leads to increased ACSL4 promoter activity, mRNA and protein expression, and consequently higher arachidonoyl-CoA synthetase activity.
27
Amhr2-Cre-Mediated Global Tspo Knockout.
Jinjiang Fan,Jinjiang Fan,Enrico Campioli,Enrico Campioli,Chantal M. Sottas,Barry R. Zirkin,Vassilios Papadopoulos,Vassilios Papadopoulos,Vassilios Papadopoulos +8 more
TL;DR: Results show that Tspo deletion causes delayed preimplantation embryonic development, alters neutral lipid storage and steroidogenesis, and leads to transcriptome changes that may reflect compensatory mechanisms in response to the loss of function of TSPO.
21
References
Inhibition of Ceramide Synthesis Ameliorates Glucocorticoid-, Saturated-Fat-, and Obesity-Induced Insulin Resistance
William L. Holland,Joseph T. Brozinick,Liping Wang,Eric D. Hawkins,Katherine M. Sargent,Yanqi Liu,Krishna K. Narra,Kyle L. Hoehn,Trina A. Knotts,Angela M. Siesky,Don H. Nelson,Sotirios K. Karathanasis,Greg K Fontenot,Morris J. Birnbaum,Scott A. Summers +14 more
TL;DR: It is demonstrated that the sphingolipid ceramide is a common molecular intermediate linking several different pathological metabolic stresses to the induction of insulin resistance, and enzymes required for ceramide synthesis are identified as therapeutic targets for combating insulin resistance caused by nutrient excess or glucocorticoid therapy.
1.2K
Physiological functions of the mitochondrial uncoupling proteins UCP2 and UCP3
Martin D. Brand,Telma C. Esteves +1 more
TL;DR: UCP2 and UCP3 are important potential targets for treatment of aging, degenerative diseases, diabetes, and perhaps obesity.
797
NASH is an Inflammatory Disorder: Pathogenic, Prognostic and Therapeutic Implications
TL;DR: Evidence that liver inflammation has prognostic significance in NAFLD is presented and the origins and components of liver inflammation in NASH are considered, along with mechanistic considerations, which project their implications for the effective treatment of NASH.
Genes Involved in Fatty Acid Partitioning and Binding, Lipolysis, Monocyte/Macrophage Recruitment, and Inflammation Are Overexpressed in the Human Fatty Liver of Insulin-Resistant Subjects
Jukka Westerbacka,Maria Kolak,Tuula Kiviluoto,Perttu Arkkila,Jukka Sirén,Anders Hamsten,Rachel M. Fisher,Hannele Yki-Järvinen +7 more
TL;DR: Genes involved in fatty acid partitioning and binding, lipolysis, and monocyte/macrophage recruitment and inflammation are overexpressed in the human fatty liver.
361
Intramembrane Proteolysis by γ-Secretase
TL;DR: Modulators of γ-secretase, which selectively affect the production of the pathological 42-amino acid Aβ, do not inhibit Notch signaling and thus cause rather significant side effects in human trials.
219
Related Papers (5)
[...]