Journal Article10.1126/SCIENCE.1191776
Micro-Optical Sectioning Tomography to Obtain a High-Resolution Atlas of the Mouse Brain
Anan Li,Hui Gong,Bin Zhang,Qingdi Wang,Cheng Yan,Jingpeng Wu,Qian Liu,Shaoqun Zeng,Qingming Luo +8 more
TL;DR: An automated system that allowed three-dimensional mapping of the morphology and spatial location of neurons and traces of neurites in a whole, intact mouse brain is described and it is found that neighboring Purkinje cells stick to each other.
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Abstract: The neuroanatomical architecture is considered to be the basis for understanding brain function and dysfunction. However, existing imaging tools have limitations for brainwide mapping of neural circuits at a mesoscale level. We developed a micro-optical sectioning tomography (MOST) system that can provide micrometer-scale tomography of a centimeter-sized whole mouse brain. Using MOST, we obtained a three-dimensional structural data set of a Golgi-stained whole mouse brain at the neurite level. The morphology and spatial locations of neurons and traces of neurites could be clearly distinguished. We found that neighboring Purkinje cells stick to each other.
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Citations
DMD-based LED-illumination Super-resolution and optical sectioning microscopy
Dan Dan,Ming Lei,Baoli Yao,Wen Wang,Martin Winterhalder,Andreas Zumbusch,Yujiao Qi,Liang Xia,Shaohui Yan,Yanlong Yang,Peng Gao,Tong Ye,Wei Zhao +12 more
TL;DR: In this paper, a DMD-based LED-illumination SIM was proposed for 3D optical microscopy, achieving a lateral resolution of 90nm and optical sectioning depth of 120μm.
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Whole-body and Whole-Organ Clearing and Imaging Techniques with Single-Cell Resolution: Toward Organism-Level Systems Biology in Mammals
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278
Morphological diversity of single neurons in molecularly defined cell types
Hanchuan Peng,Hanchuan Peng,Peng Xie,Lijuan Liu,Xiuli Kuang,Yimin Wang,Yimin Wang,Lei Qu,Lei Qu,Hui Gong,Shengdian Jiang,Anan Li,Zongcai Ruan,Liya Ding,Zizhen Yao,Chao Chen,Mengya Chen,Tanya L. Daigle,Rachel A. Dalley,Zhangcan Ding,Yanjun Duan,Aaron Feiner,Ping He,Chris Hill,Karla E. Hirokawa,Guodong Hong,Lei Huang,Sara Kebede,Hsien-Chi Kuo,Rachael Larsen,Phil Lesnar,Longfei Li,Qi Li,Xiangning Li,Yaoyao Li,Yuanyuan Li,An Liu,Donghuan Lu,Stephanie Mok,Lydia Ng,Thuc Nghi Nguyen,Qiang Ouyang,Jintao Pan,Elise Shen,Yuanyuan Song,Susan M. Sunkin,Bosiljka Tasic,Matthew B. Veldman,Wayne Wakeman,Wan Wan,Peng Wang,Quanxin Wang,Tao Wang,Yaping Wang,Feng Xiong,Wei Xiong,Wenjie Xu,Min Ye,Lulu Yin,Yang Yu,Jia Yuan,Jing Yuan,Zhixi Yun,Shaoqun Zeng,Shichen Zhang,Sujun Zhao,Zijun Zhao,Zhi Zhou,Z. Josh Huang,Z. Josh Huang,Luke Esposito,Michael Hawrylycz,Staci A. Sorensen,X. William Yang,Yefeng Zheng,Zhongze Gu,Wei Xie,Christof Koch,Qingming Luo,Qingming Luo,Julie A. Harris,Yun Wang,Hongkui Zeng +82 more
TL;DR: In this paper, the authors systematically examined complete single-neuron morphologies on a brain-wide scale, established a pipeline encompassing sparse labeling, whole-brain imaging, reconstruction, registration and analysis, and identified 11 major projection neuron types with distinct morphological features and corresponding transcriptomic identities.
Continuously tracing brain-wide long-distance axonal projections in mice at a one-micron voxel resolution.
Hui Gong,Shaoqun Zeng,Cheng Yan,Xiaohua Lv,Zhongqin Yang,Tonghui Xu,Zhao Feng,Wenxiang Ding,Xiaoli Qi,Anan Li,Jingpeng Wu,Qingming Luo +11 more
TL;DR: A whole-brain approach to systematically obtaining continuous neuronal pathways in a fluorescent protein transgenic mouse at a one-micron voxel resolution is described, believed to open an avenue to exploring both local and long-distance neural circuits that are related to brain functions and brain diseases down to the neurite level.
259
Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse Brain.
Benyi Xiong,Anan Li,Yang Lou,Shangbin Chen,Ben Long,Jie Peng,Zhongqin Yang,Tonghui Xu,Xiaoquan Yang,Xiangning Li,Tao Jiang,Qingming Luo,Hui Gong +12 more
TL;DR: Reconstruction and statistical analysis of the microvascular network, including derivation of quantitative vascular densities, indicate significant differences mainly in vessels with diameters less than 8 μm and large than 20 μm across different brain regions.
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