Open AccessJournal Article
Methylation microarray analysis of late-stage ovarian carcinomas distinguishes progression-free survival in patients and identifies candidate epigenetic markers
Susan H. Wei,Chuan-Mu Chen,Gordon Strathdee,Jaturon Harnsomburana,Chi-Ren Shyu,Farahnaz Rahmatpanah,Huidong Shi,Shu Wing Ng,Pearlly S. Yan,Kenneth P. Nephew,Robert S. Brown,Tim H M Huang +11 more
TL;DR: It is suggested that a higher degree of CpG island methylation is associated with early disease recurrence after chemotherapy, and this degree is potentially useful as epigenetic markers for predicting treatment outcome in ovarian cancer patients.
read more
Abstract: Purpose: The purpose of this study was to profile methylation alterations of CpG islands in ovarian tumors and to identify candidate markers for diagnosis and prognosis of the disease.
Experimental Design: A global analysis of DNA methylation using a novel microarray approach called differential methylation hybridization was performed on 19 patients with stage III and IV ovarian carcinomas.
Results: Hierarchical clustering identified two groups of patients with distinct methylation profiles. Tumors from group 1 contained high levels of concurrent methylation, whereas group 2 tumors had lower tumor methylation levels. The duration of progression-free survival after chemotherapy was significantly shorter for patients in group 1 compared with group 2 ( P < 0.001). Differential methylation in tumors was independently confirmed by methylation-specific PCR.
Conclusions: The data suggest that a higher degree of CpG island methylation is associated with early disease recurrence after chemotherapy. The differential methylation hybridization assay also identified a select group of CpG island loci that are potentially useful as epigenetic markers for predicting treatment outcome in ovarian cancer patients.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
CpG-island methylation and epigenetic control of resistance to chemotherapy
TL;DR: Clinical trials of epigenetic therapies are now in progress, and epigenetic profiling using DNA methylation will provide guidance on optimization of the use of these therapies with conventional chemotherapy, as well as helping to identify patient populations who may particularly benefit from such approaches.
41
Ovarian cancer plasticity and epigenomics in the acquisition of a stem-like phenotype.
TL;DR: To illuminate phenotypic signatures that delineate ovarian cancer from their associated cancer stem cells, a priority must lie in the expansion of current technologies and further implementation of bioinformatics to handle the complexity of the cancer epigenome and the various networks that coordinate disease initiation and progression.
Differential methylation hybridization array of endometrial cancers reveals two novel cancer-specific methylation markers.
Israel Zighelboim,Paul J. Goodfellow,Amy P. Schmidt,Ken C. Walls,Mary Ann Mallon,David G. Mutch,Pearlly S. Yan,Tim H M Huang,Matthew A. Powell +8 more
TL;DR: SESN3, TITF1 and MLH1 methylation did not predict overall survival or disease-free survival in this large cohort of patients with endometrioid endometrial cancer.
40
Aberrant DNA Methylation in Ovarian Cancer
TL;DR: Results indicate that loss of gene activity due to hypermethylation potentially confers a predisposition in certain cancer types and is an early event in disease progression.
39
Aberrant DNA methylation profile and frequent methylation of KLK10 and OXGR1 genes in hepatocellular carcinoma
Chang Yi Lu,Sen Yung Hsieh,Yen Jung Lu,Chi Sheng Wu,Lih Chyang Chen,Shao Jung Lo,Cheng Tao Wu,Cheng Tao Wu,Min Yuan Chou,Tim H M Huang,Yu-Sun Chang +10 more
TL;DR: The results suggest that the hypermethylated KLK10 and OXGR1 are frequent in HCC and may be useful as markers for clinical application.
36
References
Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands
TL;DR: The use of MSP is demonstrated to identify promoter region hypermethylation changes associated with transcriptional inactivation in four important tumor suppressor genes (p16, p15, E-cadherin and von Hippel-Lindau) in human cancer.
6.1K
Cancer statistics, 2001.
TL;DR: Estimates of the number of new cancer cases and deaths expected in the US in the current year and the most recent data on cancer incidence, mortality, and survival reveal large disparities in cancer incidence and mortality across racial/ethnic groups.
3.5K
Cancer-epigenetics comes of age
Peter A. Jones,Peter W. Laird +1 more
TL;DR: Current mechanistic understanding of the role of DNA methylation in malignant transformation is reviewed, and it is suggested Knudson's two–hit hypothesis should be expanded to include epigenetic mechanisms of gene inactivation.
2.5K
Aberrant patterns of DNA methylation, chromatin formation and gene expression in cancer
Stephen B. Baylin,Manel Esteller,Michael R. Rountree,Kurtis E. Bachman,Kornel E. Schuebel,James G. Herman +5 more
TL;DR: In the translational arena, the promoter hypermethylation changes hold great promise as DNA tumor markers and their potentially reversible state creates a target for cancer therapeutic strategies involving gene reactivation.
•Journal Article
Reversal of drug resistance in human tumor xenografts by 2'-deoxy-5-azacytidine-induced demethylation of the hMLH1 gene promoter.
TL;DR: DAC could have a role in increasing the efficacy of chemotherapy for patients whose tumors lack MLH1 expression because of hMLH1 promoter methylation, and is consistent with loss of MMR having no effect on sensitivity in vitro to Taxol.
531
Related Papers (5)
Joseph F. Costello,Joseph F. Costello,Michael C. Frühwald,Michael C. Frühwald,Dominic J. Smiraglia,Laura J. Rush,Gavin P. Robertson,Xin Gao,Fred A. Wright,Jamison D. Feramisco,Päivi Peltomäki,James C. Lang,David E. Schuller,Li Yu,Clara D. Bloomfield,Michael A. Caligiuri,Allan J. Yates,Ryo Nishikawa,H.-J. Su Huang,Nicholas J. Petrelli,Xueli Zhang,M. S. O'Dorisio,William A. Held,Webster K. Cavenee,Webster K. Cavenee,Christoph Plass +25 more