Method Development and Validation for Simultaneous Estimation of Ethinyl Estradiol and Drospirenone and Forced Degradation Behavior by HPLC in Combined Dosage Form
Praveen C,Ranganath Mk,Divakar P +2 more
TL;DR: In this paper, a simple, accurate, rapid and precise isocratic high performance liquid chromatographic (HPLC) method was developed and validated for the determination of ethinyl estradiol and drospirenone in tablet formulation.
read more
Abstract: A simple, accurate, rapid and precise isocratic High performance liquid chromatographic (HPLC) method was developed and validated for the determination of ethinyl estradiol and drospirenone in tablet formulation. The method employs Waters HPLC system on Thermo Hypersil BDS C18 Column (4.6×250 mm and 5 μm) and flow rate of 1.0 ml/min with a load of 15 μl. Acetonitrile and ammonium acetate buffer was used as mobile phase in the composition of 30:70. The detection was carried out at 258 nm. Linearity ranges for ethinyl estradiol and drospirenone were 0.06- 0.18 μg/ml, 6-18 μg/ml respectively. Retention Time of ethinyl estradiol and drospirenone were found to be 1.4 min, 5.3 min respectively. Percent Recovery study values of ethinyl estradiol and drospirenone were found to be within 97-103%. The combination product is exposed to acid/base, hydrolytic, photolytic and peroxide stress conditions and the stressed samples were analyzed. This developed method was successfully utilized for the quantitative estimation of ethinyl estradiol and drospirenone in pharmaceutical dosage forms. This method was validated for accuracy, precision, linearity and Robustness as per ICH guidelines.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
A Review on Chromatography with High Performance Liquid Chromatography (HPLC) and its Functions
Sneha Lakshmi R.P
- 04 Jun 2015
TL;DR: In this paper, a mobile phase is proposed for determination of Metadoxine and Atazanavir from pure and its dosage forms, which can also be used for dissolution or similar studies.
Development and validation of stability indicating rp-hplc method for simultaneous estimation of ethinyl estradiol and levonorgestrel
Tejal Rathod,Anandkumari D. Captain +1 more
- 01 Jan 2014
TL;DR: In this paper, a simple, accurate, precise, sensitive, specific and reliable stability indicating RP-HPLC method was developed for simultaneous estimation of Ethinyl estradiol (EE) and Levonorgestrel (LEV) in Pharmaceutical dosage form.
Detection of 1,4-Benzodiazepine by Using Different Analytical Methods
Anupama P
- 01 Jan 2016
TL;DR: Benzodiazepines are generally utilized as a hostile to depressive and narcotic medications, furthermore as against epileptic medications and now and again can be valuable as an assistant treatment in headstrong epilepsies or hostile to alcoholic treatment.
Novel reversed-phase HPLC method for simultaneous determination of ethynodiol diacetate (EDA)/ethinyl estradiol (EE) in pharmaceutical dosage form
Srinivasa Reddy Chinta,Vijetha Ponnam,Naresh Kumar Katari,Sasikiran Goud Ediga,Vishnu Murthy Marisetti +4 more
TL;DR: In this paper, the linearity and recovery were found in the range of 0.025-0.25 mg/mL and 0.05- 0.18 mg/dL, respectively, for EDA and EE, respectively.
2
Development and validation of a liquid chromatography/tandem mass spectrometric method for determination of ethinyl estradiol in human plasma
Vijay Kotra,Nageswara Rao Ramisetti,Rajeesha Surapaneni,Sathish Kumar Konidala +3 more
- 01 Jan 2017
TL;DR: A simple and rapid bioanalytical liquid chromatography-tandem mass spectrometry (LC-MS/MS) method based on solid phase extraction followed by liquid-liquid extraction (LLE) has been developed and validated for quantification of Ethinyl Estradiol in human plasma and was validated as per ICH guidelines.
2
References
•Book
Martindale : the complete drug reference
Alison Brayfield
- 01 Jan 2014
TL;DR: This 38th edition of Martindale has been completely redesigned to ensure ease of use and greatly improved readability.
1.2K
Pharmacokinetic and pharmacodynamic alterations of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors: Drug–drug interactions and interindividual differences in transporter and metabolic enzyme functions
TL;DR: This review presents a quantitative analysis of the effect of some factors on the pharmacokinetics of statins based on a physiologically based pharmacokinetic model and suggests that model-based prediction is also useful.
589
Martindale: The Complete Drug Reference
TL;DR: The 32nd edition of Martindale differs from earlier ones not only by its title—it is no longer ‘The Extra Pharmacopoiea’ but ‘the Complete Drug Reference’, and each of its 5000 monographs now incorporates a section on Interactions, ensuring this information is readily accessible.
353
A stability-indicating high performance liquid chromatographic (HPLC) assay for the simultaneous determination of atorvastatin and amlodipine in commercial tablets.
Ali Mohammadi,N. Rezanour,M. Ansari Dogaheh,F. Ghorbani Bidkorbeh,M. Hashem,Roderick B. Walker +5 more
TL;DR: A simple, rapid, precise and accurate isocratic reversed-phase stability-indicating HPLC method was developed and validated for the simultaneous determination of atorvastatin (AT) and amlodipine (AM) in commercial tablets and has shown adequate separation for AM, AT from their associated main impurities and their degradation products.
194