Metabolomics analysis of urine from rats administered with long-term, low-dose acrylamide by ultra-performance liquid chromatography-mass spectrometry.
TL;DR: Exposure to AA disrupts the metabolism of lipids and amino acids, induces oxidative stress, and three new candidate biomarkers in rat urine exposed to AA have been found in this study.
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Abstract: 1. To study the toxic effect of chronic exposure to acrylamide (AA) at low-dose levels, we applied metabolomics approach based on ultra-performance liquid chromatography/mass spectrometry (UPLC–MS). A total of 40 male Wistar rats were randomly assigned to different groups: control, low-dose AA (0.2 mg/kg.bw), middle-dose AA (1 mg/kg.bw) and high-dose AA (5 mg/kg.bw). The rats continuously received AA via drinking water for 16 weeks. Rat urine samples were collected at different time points for measurement of metabolomic profiles.2. Thirteen metabolites, including the biomarkers of AA exposure (AAMA, GAMA and iso-GAMA), were identified from the metabolomic profiles of rat urine. Compared with the control group, the treated groups showed significantly increased intensities of GAMA, AAMA, iso-GAMA, vinylacetylglycine, 1-salicylate glucuronide, PE (20:1(11Z)/14:0), cysteic acid, L-cysteine, p-cresol sulfate and 7-ketodeoxycholic acid, as well as decreased intensities of 3-acetamidobutanal, 2-indolecar...
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Acrylamide Induced Toxicity and the Propensity of Phytochemicals in Amelioration: A Review.
TL;DR: Various toxicities that are induced due to acrylamide, its proposed mode of action including oxidative stress cascades and ameliorative mechanisms using phytochemicals, and the studies on the role of oxidative stress and mitochondrial dysfunctions are highlighted.
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Serum Metabolomics Analysis of Quercetin against Acrylamide-Induced Toxicity in Rats.
TL;DR: Results indicate that quercetin (50 mg/kg bw) exerts partial protective effects on AA-induced toxicity by reducing oxidative stress, protecting the mitochondria, and regulating lipid metabolism.
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Acrylamide in food: Occurrence, metabolism, molecular toxicity mechanism and detoxification by phytochemicals.
TL;DR: In this paper , a review summarizes the ACR level in foods and its metabolic pathways, as well as highlights the mechanisms underlying ACR-induced toxicity and ACR detoxification by phytochemicals.
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Metabonomics analysis of quercetin against the nephrotoxicity of acrylamide in rats.
TL;DR: The results showed that quercetin exerted protective effects on AA-induced nephrotoxicity by enhancing the antioxidant defence system of the body and regulating phospholipid, energy and amino acid metabolism.
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Metabonomic analysis of quercetin against the toxicity of acrylamide in rat urine.
TL;DR: The above results suggest that quercetin has a partial protective effect on AA-induced toxicity, which includes regulation of fatty acid metabolism and amino acids metabolism and enhancing the antioxidant defense system.
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