Metabolite recognition principles and molecular mechanisms underlying riboswitch function.
TL;DR: Three-dimensional information supplemented by structure-guided biophysical and biochemical experimentation has led to an improved understanding of how riboswitches fold, what RNA conformations are required for ligand recognition, and how ligand binding can be transduced into gene expression modulation.
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Abstract: Riboswitches are mRNA elements capable of modulating gene expression in response to specific binding by cellular metabolites. Riboswitches exert their function through the interplay of alternative ligand-free and ligand-bound conformations of the metabolite-sensing domain, which in turn modulate the formation of adjacent gene expression controlling elements. X-ray crystallography and NMR spectroscopy have determined three-dimensional structures of virtually all the major riboswitch classes in the ligand-bound state and, for several riboswitches, in the ligand-free state. The resulting spatial topologies have demonstrated the wide diversity of riboswitch folds and revealed structural principles for specific recognition by cognate metabolites. The available three-dimensional information, supplemented by structure-guided biophysical and biochemical experimentation, has led to an improved understanding of how riboswitches fold, what RNA conformations are required for ligand recognition, and how ligand binding...
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Citations
A triple helix stabilizes the 3′ ends of long noncoding RNAs that lack poly(A) tails
Jeremy E. Wilusz,Courtney K. JnBaptiste,Laura Lu,Claus-D. Kuhn,Leemor Joshua-Tor,Leemor Joshua-Tor,Phillip A. Sharp +6 more
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TL;DR: It is predicted that there are potentially many thousands of distinct bacterial riboswitch classes remaining to be discovered, but that the rarity of individual undiscovered classes will make it increasingly difficult to find additional examples of this RNA-based sensory and gene control mechanism.
Regulation of Bacterial Virulence by Csr (Rsm) Systems
TL;DR: The workings of the Csr system, the paradigm shift that it generated for understanding posttranscriptional regulation, and its roles in virulence networks of animal and plant pathogens are presented.
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Amin Espah Borujeni,Dennis M. Mishler,Jingzhi Wang,Walker Huso,Howard M. Salis,Howard M. Salis +5 more
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Hierarchy of RNA functional dynamics
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Metals, Motifs, and Recognition in the Crystal Structure of a 5S rRNA Domain
Carl C. Correll,Carl C. Correll,B. Freeborn,Peter B. Moore,Thomas A. Steitz,Thomas A. Steitz +5 more
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Miyun Kwon,Scott A. Strobel +1 more
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Elaine R. Lee
- 01 Jan 2010
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