Mechanism of the chilling-induced decrease in proton pumping across the tonoplast of rice cells.
TL;DR: The ATP-generated proton pumping across tonoplast vesicles from chilling-sensitive Boro rice (Oryza sativa L. var. Boro) cultured cells was markedly decreased by chilling, suggesting that the reduction is due to the decrease in the fluidity of the surface region of the lipid bilayer of the tonopleft, which is caused by the changes in glycolipids.
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Abstract: The ATP-generated proton pumping across tonoplast vesicles from chilling-sensitive Boro rice (Oryza sativa L. var. Boro) cultured cells was markedly decreased by chilling at 5 degrees C for 3 d. The membrane fluidity of core hydrophobic and surface hydrophilic regions of the lipid bilayer was measured by steady-state fluorescence depolarization of 1,6-diphenyl-1,3,5-hexatriene and trimethylammonium 1,6-diphenyl-1,3,5-hexatriene and by electron spin resonance spectroscopy of 16- and 5-doxyl stearic acid, respectively. The fluidity of the surface region of the lipid bilayer of the tonoplast vesicles decreased by chilling. The fluidity of the surface region of the liposomes and the proton pumping across the reconstituted proteoliposomes with tonoplast H+-ATPase decreased with increasing content of the glycolipids. The proton pumping across chimera proteoliposomes was reduced by chilling only when it was reconstituted in the presence of tonoplast glycolipids from chilled Boro cells. These data suggest that the reduction in ATP-generated proton pumping across the tonoplast by chilling is due to the decrease in the fluidity of the surface region of the lipid bilayer of the tonoplast, which is caused by the changes in glycolipids.
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Significance of the V‐type ATPase for the adaptation to stressful growth conditions and its regulation on the molecular and biochemical level
Karl-Josef Dietz,Nastaran Tavakoli,Christoph Kluge,Tetsuro Mimura,Shanti S. Sharma,Gary C. Harris,A. N. Chardonnens,Dortje Golldack +7 more
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New insight into the structure and regulation of the plant vacuolar H+-ATPase.
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TL;DR: The review summarizes recent advances in elucidating the structure, subunit composition, localization, and regulation of plant V-ATPase.
100
Changes in freezing tolerance, plasma membrane H+-ATPase activity and fatty acid composition in Pinus resinosa needles during cold acclimation and de-acclimation.
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TL;DR: A comparative proteomic analysis between tomato fruits stored at chilling and non-chilling temperatures was carried out just before the appearance of visible symptoms of chilling injury, suggesting aldose-1-epimerase, which seems to have an important role in low temperature tolerance, may be used as a protein marker for further studies on cold stress.
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