Journal Article10.1002/ANIE.200503873
Materials for Fluorescence Resonance Energy Transfer Analysis: Beyond Traditional Donor-Acceptor Combinations
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TL;DR: This review gives a critical overview of the major classes of fluorophore materials that may act as donor, acceptor, or both in a FRET configuration and focuses on the benefits and limitations of these materials and their combinations, as well as the available methods of bioconjugation.
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Abstract: The use of Forster or fluorescence resonance energy transfer (FRET) as a spectroscopic technique has been in practice for over 50 years. A search of ISI Web of Science with just the acronym "FRET" returns more than 2300 citations from various areas such as structural elucidation of biological molecules and their interactions, in vitro assays, in vivo monitoring in cellular research, nucleic acid analysis, signal transduction, light harvesting and metallic nanomaterials. The advent of new classes of fluorophores including nanocrystals, nanoparticles, polymers, and genetically encoded proteins, in conjunction with ever more sophisticated equipment, has been vital in this development. This review gives a critical overview of the major classes of fluorophore materials that may act as donor, acceptor, or both in a FRET configuration. We focus in particular on the benefits and limitations of these materials and their combinations, as well as the available methods of bioconjugation.
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References
•Book
Principles of fluorescence spectroscopy
Joseph R. Lakowicz
- 01 Jan 1983
TL;DR: This book describes the fundamental aspects of fluorescence, the biochemical applications of this methodology, and the instrumentation used in fluorescence spectroscopy.
Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology.
TL;DR: A review of gold nanoparticles can be found in this article, where the most stable metal nanoparticles, called gold colloids (AuNPs), have been used for catalysis and biology applications.
12.6K
Semiconductor Nanocrystals as Fluorescent Biological Labels
TL;DR: Semiconductor nanocrystals prepared for use as fluorescent probes in biological staining and diagnostics have a narrow, tunable, symmetric emission spectrum and are photochemically stable.
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Zwischenmolekulare Energiewanderung und Fluoreszenz
TL;DR: In this article, a quantenmechanische behandlung des Ubergangs von Elektronenanregungsenergie zwischen gleichartigen Molekulen in Losung gegeben.
8.2K
Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics
Xavier Michalet,Fabien Pinaud,Laurent A. Bentolila,James M. Tsay,Sören Doose,J. Jack Li,Gobalakrishnan Sundaresan,Anna M. Wu,Sanjiv S. Gambhir,Sanjiv S. Gambhir,Shimon Weiss +10 more
TL;DR: The new generations of qdots have far-reaching potential for the study of intracellular processes at the single-molecule level, high-resolution cellular imaging, long-term in vivo observation of cell trafficking, tumor targeting, and diagnostics.