Major latex protein-like protein 43 (MLP43) functions as a positive regulator during abscisic acid responses and confers drought tolerance in Arabidopsis thaliana
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TL;DR: MLP43 interacts with SnRK2.6 and ABF1 and functions as a positive regulator in ABA and drought stress responses through modulating gene expression, ROS production and primary metabolite profiles.
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Abstract: Drought stress is one of the disadvantageous environmental conditions for plant growth and reproduction. Given the importance of abscisic acid (ABA) to plant growth and abiotic stress responses, identification of novel components involved in ABA signalling transduction is critical. In this study, we screened numerous Arabidopsis thaliana mutants by seed germination assay and identified a mutant mlp43 (major latex protein-like 43) with decreased ABA sensitivity in seed germination. The mlp43 mutant was sensitive to drought stress while the MLP43-overexpressed transgenic plants were drought tolerant. The tissue-specific expression pattern analysis showed that MLP43 was predominantly expressed in cotyledons, primary roots and apical meristems, and a subcellular localization study indicated that MLP43 was localized in the nucleus and cytoplasm. Physiological and biochemical analyses indicated that MLP43 functioned as a positive regulator in ABA- and drought-stress responses in Arabidopsis through regulating water loss efficiency, electrolyte leakage, ROS levels, and as well as ABA-responsive gene expression. Moreover, metabolite profiling analysis indicated that MLP43 could modulate the production of primary metabolites under drought stress conditions. Reconstitution of ABA signalling components in Arabidopsis protoplasts indicated that MLP43 was involved in ABA signalling transduction and acted upstream of SnRK2s by directly interacting with SnRK2.6 and ABF1 in a yeast two-hybrid assay. Moreover, ABA and drought stress down-regulated MLP43 expression as a negative feedback loop regulation to the performance of MLP43 in ABA and drought stress responses. Therefore, this study provided new insights for interpretation of physiological and molecular mechanisms of Arabidopsis MLP43 mediating ABA signalling transduction and drought stress responses.
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Citations
Abscisic Acid and Abiotic Stress Tolerance in Crop Plants
TL;DR: The role of ABA in response to abiotic stress at the molecular level and ABA signaling is discussed and the effect of A BA in respect to gene expression is dealt with.
A Functional Genomic Perspective on Drought Signalling and its Crosstalk with Phytohormone-mediated Signalling Pathways in Plants.
TL;DR: The molecular mechanism of drought signalling and its crosstalk with various phytohormone signalling pathways implicated in abiotic stress response and tolerance is summarized.
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Abscisic acid: A key regulator of abiotic stress tolerance in plants
Niyaz A. Dar,Insha Amin,Wasia Wani,Shafiq A. Wani,Asif B. Shikari,Shabir H. Wani,Khalid Z. Masoodi +6 more
TL;DR: The role of transcription factors that regulate ABA signal transduction in response to abiotic stress are discussed in order to discuss the role of stress-responsive genes in plants under stress.
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A B-box zinc finger protein, MdBBX10, enhanced salt and drought stresses tolerance in Arabidopsis
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RNA-seq Transcriptome Response of Flax (Linum usitatissimum L.) to the Pathogenic Fungus Fusarium oxysporum f. sp. lini.
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