Macrophage-Derived Extracellular Vesicle Promotes Hair Growth
Ramya Lakshmi Rajendran,Prakash Gangadaran,Prakash Gangadaran,Chang Hoon Seo,Mi Hee Kwack,Ji Min Oh,Ji Min Oh,Ho-Won Lee,Ho-Won Lee,Arunnehru Gopal,Arunnehru Gopal,Young Kwan Sung,Shin Young Jeong,Shin Young Jeong,Sang-Woo Lee,Sang-Woo Lee,Jaetae Lee,Jaetae Lee,Byeong-Cheol Ahn,Byeong-Cheol Ahn +19 more
64
TL;DR: The findings suggest that MAC-EV treatment could be clinically used as a promising novel anagen inducer in the treatment of hair loss.
read more
Abstract: Hair loss is a common medical problem affecting both males and females. Dermal papilla (DP) cells are the ultimate reservoir of cells with the potential of hair regeneration in hair loss patients. Here, we analyzed the role of macrophage-derived Wnts (3a and 7b) and macrophage extracellular vesicles (MAC-EVs) in promoting hair growth. We studied the proliferation, migration, and expression of growth factors of human-DP cells in the presence or absence of MAC-EVs. Additionally, we tested the effect of MAC-EV treatment on hair growth in a mouse model and human hair follicles. Data from western blot and flow cytometry showed that MAC-EVs were enriched with Wnt3a and Wnt7b, and more than 95% were associated with their membrane. The results suggest that Wnt proteins in MAC-EVs activate the Wnt/β-catenin signaling pathways, which leads to activation of transcription factors (Axin2 and Lef1). The MAC-EVs significantly enhanced the proliferation, migration, and levels of hair-inductive markers of DP cells. Additionally, MAC-EVs phosphorylated AKT and increased the levels of the survival protein Bcl-2. The DP cells treated with MAC-EVs showed increased expression of vascular endothelial growth factor (VEGF) and keratinocyte growth factor (KGF). Treatment of Balb/c mice with MAC-EVs promoted hair follicle (HF) growth in vivo and also increased hair shaft size in a short period in human HFs. Our findings suggest that MAC-EV treatment could be clinically used as a promising novel anagen inducer in the treatment of hair loss.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Macrophage-derived extracellular vesicles: diverse mediators of pathology and therapeutics in multiple diseases
TL;DR: An overview of macrophage and EV biology is introduced, primarily focusing on current findings and future perspectives with respect to the pathological and therapeutic effects of Mφ-EVs in various diseases.
CAR T-Cell-Based gene therapy for cancers: new perspectives, challenges, and clinical developments
Manasi P Jogalekar,Ramya Lakshmi Rajendran,Fatima Khan,Crismita Dmello,Prakash Gangadaran,Byeong-Cheol Ahn +5 more
TL;DR: This review briefly discusses cancer immunotherapy and the genetic engineering of T cells and, in detail, the current innovations in CAR T-cell strategies to improve efficacy in treating solid tumors and hematologic malignancies.
A systematic summary of survival and death signalling during the life of hair follicle stem cells.
Xi-Min Hu,Zhi-Xin Li,Dan-Yi Zhang,Yi-Chao Yang,Shen-Ao Fu,Zai-Qiu Zhang,Ronghua Yang,Kun Xiong +7 more
TL;DR: The potential mechanisms underlying the therapeutic targets involved in pathways associated with the treatment of hair loss and other disorders of skin and hair, including alopecia, skin cancer, skin inflammation, and skin wound healing are highlighted.
Regenerative medicine strategies for hair growth and regeneration: A narrative review of literature
TL;DR: In this article , the most promising regenerative treatments for alopecia were summarized and further opportunities of improving efficacy and innovative strategies for promoting clinical application were discussed, with increasing evidence suggesting that mesenchymal stem cell (MSC) implantation, MSC-derived secretome treatment, and blood-derived platelet-rich plasma therapies are potential treatment options.
63
Chemically Engineered Immune Cell-Derived Microrobots and Biomimetic Nanoparticles: Emerging Biodiagnostic and Therapeutic Tools
Leila Pourtalebi Jahromi,Mohammad-Ali Shahbazi,Mohammad-Ali Shahbazi,Aziz Maleki,Amir Azadi,Hélder A. Santos +5 more
TL;DR: In this article, the latest progress in the development of engineered therapeutics and diagnostics inspired by immune cells to ameliorate cancer, inflammatory conditions, autoimmune diseases, neurodegenerative disorders, cardiovascular complications, and infectious diseases is reviewed, and finally, the perspective for their clinical application is delineated.
58
References
Wnt proteins are lipid-modified and can act as stem cell growth factors
Karl Willert,Jeffrey Brown,Esther Danenberg,Andrew W. Duncan,Irving L. Weissman,Tannishtha Reya,John R. Yates,Roel Nusse +7 more
TL;DR: This work isolated active Wnt molecules, including the product of the mouse Wnt3a gene, and found the proteins to be palmitoylated on a conserved cysteine, indicating that the lipid is important for signalling.
The Bcl-2 family: roles in cell survival and oncogenesis.
TL;DR: Current views of how the Bcl-2 family of anti- and proapoptotic regulators sense stress, interact with their relatives, perturb organelles such as the mitochondrion and endoplasmic reticulum and govern pathways to caspase activation are summarized.
1.6K
Wnt Signaling: Multiple Pathways, Multiple Receptors, and Multiple Transcription Factors
Michael D. Gordon,Roel Nusse +1 more
TL;DR: The majority of work in the field to date has focused on -catenin-dependent, or canonical, Wnt signaling, but examples continue to accumulate in which Wnts and/or other key components of the canonical signaling cascade participate in -catanin-independent processes.
1.4K
beta-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin.
TL;DR: It is shown that beta-catenin is required genetically downstream of tabby/downless and upstream of bmp and shh in placode formation and is essential for fate decisions of skin stem cells: in the absence of beta-Catenin, stem cells fail to differentiate into follicular keratinocytes, but instead adopt an epidermal fate.
1.4K
Active Wnt proteins are secreted on exosomes
TL;DR: It is demonstrated that exosomes carry Wnts on their surface to induce Wnt signalling activity in target cells and an evolutionarily conserved functional role of extracellular vesicular transport of Wnt proteins is demonstrated.
984