Journal Article10.1016/j.isci.2023.107491
Low-oxygen tension augments chondrocyte sensitivity to biomimetic thermomechanical cues in cartilage-engineered constructs
Theofanis Stampoultzis,Yanheng Guo,Naser Nasrollahzadeh,Vijay Kumar Rana,Peyman Karami,Dominique P. Pioletti +5 more
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TL;DR: This mechanistic study highlighted the crucial role of SRY-box transcription factor 9 (SOX9) as a major regulator of chondrogenic response, specifically expressed in response to combined biophysical signals, and illuminated the integration of various mechanobiological cues by chondrocytes.
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Abstract: Chondrocytes respond to various biophysical cues, including oxygen tension, transient thermal signals, and mechanical stimuli. However, understanding how these factors interact to establish a unique regulatory microenvironment for chondrocyte function remains unclear. Herein, we explore these interactions using a joint-simulating bioreactor that independently controls the culture's oxygen concentration, evolution of temperature, and mechanical loading. Our analysis revealed significant coupling between these signals, resulting in a remarkable ∼14-fold increase in collagen type II (COL2a) and aggrecan (ACAN) mRNA expression. Furthermore, dynamic thermomechanical stimulation enhanced glycosaminoglycan and COL2a protein synthesis, with the magnitude of the biosynthetic changes being oxygen dependent. Additionally, our mechanistic study highlighted the crucial role of SRY-box transcription factor 9 (SOX9) as a major regulator of chondrogenic response, specifically expressed in response to combined biophysical signals. These findings illuminate the integration of various mechanobiological cues by chondrocytes and provide valuable insights for improving the extracellular matrix content in cartilage-engineered constructs.
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Citations
Impact of Molecular Dynamics of Polyrotaxanes on Chondrocytes in Double-Network Supramolecular Hydrogels under Physiological Thermomechanical Stimulation
Theofanis Stampoultzis,Vijay Kumar Rana,Yanheng Guo,Dominique P. Pioletti +3 more
TL;DR: Double-network supramolecular hydrogels enhance chondrocyte mechanosensitivity and matrix remodeling through enhanced molecular dynamics.
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Impact of Molecular Dynamics of Polyrotaxanes on Chondrocytes in Double Network Supramolecular Hydrogels under Physiological Thermomechanical Stimulation
Theofanis Stampoultzis,Vijay Kumar Rana,Yanheng Guo,Dominique P. Pioletti +3 more
TL;DR: It is found that matrix adaptability plays a pivotal role in modulating chondrocyte responses within double-network supramolecular hydrogels, and these findings hold potential for advancing cartilage engineering within load-bearing joints.
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Biomimetic ECM-Based Hybrid Scaffold for Cartilage Tissue Engineering Applications
Davood Yari,Jebrail Movaffagh,Mohammad Ali Ebrahimzadeh,Ali Saberi,Durdi Qujeq,Ali Moradi +5 more
Impact of Tibetan ethnicity and residence altitude on complications during total knee arthroplasty and difficulties of measurement of perioperative blood loss
Wenyu Jiang,Hong Xu,Xing Liu,Huansheng Liu,Yucan Ju,Jinwei Xie,Qiang Huang,Zeyu Huang,Fuxing Pei +8 more
Thermomechanobiology as a new research field in soft tissues
Dominique P. Pioletti
TL;DR: Researchers propose "thermomechanobiology" as a new field, exploring the impact of temperature variations induced by mechanical loading on soft tissues, revealing self-heating as a crucial factor in cartilage maintenance and musculoskeletal homeostasis.
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