Long Noncoding RNA Expression Rofiles Elucidate the Potential Roles of lncRNA- XR_003496198 in Duck Hepatitis A Virus Type 1 Infection
Nana Sui,Ruihua Zhang,Yue Jiang,Honglei Yu,Guige Xu,Jingyu Wang,Yanli Zhu,Zhijing Xie,Jiaqing Hu +8 more
TL;DR: This study comprehensively analyzed the lncRNA profiles upon DHAV-1 infection and screened the target genes involved in the innate immune response and autophagy signaling pathway, thereby revealing the essential roles of duck lncRNAs and broadening the understanding of host-virus interactions.
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Abstract: Duck hepatitis A virus type 1 (DHAV-1) is a highly lethal virus that severely affects the duck industry worldwide. Long noncoding RNAs (lncRNAs) exert crucial roles in pathogen attacks. Here, we conducted deep transcriptome analysis to investigate the dynamic changes of host lncRNAs profiles in DHAV-1-infected duck embryo fibroblasts. We identified 16,589 lncRNAs in total and characterized their genomic features. Moreover, 772 and 616 differentially expressed lncRNAs (DELs) were screened at 12 and 24 h post-infection. Additionally, we predicted the DELs’ cis- and trans-target genes and constructed lncRNA-target genes regulatory networks. Functional annotation analyses indicated that the putative target genes of DELs participated in diverse vital biological processed, including immune responses, cellular metabolism, and autophagy. For example, we confirmed the dysregulation of pattern recognition receptors (TLR3, RIG-I, MDA5, LGP2, cGAS), signal transducers (STAT1), transcription factors (IRF7), immune response mediators (IL6, IL10, TRIM25, TRIM35, TRIM60, IFITM1, IFITM3, IFITM5), and autophagy-related genes (ULK1, ULK2, EIF4EBP2) using RT-qPCR. Finally, we confirmed that one DHAV-1 induced lncRNA-XR_003496198 is likely to inhibit DHAV-1 replication in DEFs. Our study comprehensively analyzed the lncRNA profiles upon DHAV-1 infection and screened the target genes involved in the innate immune response and autophagy signaling pathway, thereby revealing the essential roles of duck lncRNAs and broadening our understanding of host-virus interactions.
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Current status and future direction of duck hepatitis A virus vaccines
TL;DR: Wang et al. as discussed by the authors provided essential information for vaccine development and disease control of Duck Viral Hepatitis (DVH), mainly caused by Duck hepatitis A virus (DHAV), which seriously jeopardizes the duck industry worldwide.
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RNA-seq and microRNA association analysis to explore the pathogenic mechanism of DHAV-1 infection with DEHs
TL;DR: In this article , the mRNA and microRNA expression profiles of duck embryonic hepatocytes (DEHs) in response to DHAV-1 were analyzed and enriched utilizing GO and KEGG, which may provide a hint for the interactions of virus and host.
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Advances in the Duck Hepatitis A virus and lessons learned from those in recent years
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TL;DR: This review summarizes the molecular biology, epidemiology, and control of Duck Hepatitis A virus (DHAV), highlighting recent challenges due to mutations, genotype 3 outbreaks, and mixed infections, providing a scientific basis for future research and disease management.
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TL;DR: This study reveals that Lnc BTU upregulation by duck plague virus (DPV) promotes DNA polymerase production and inhibits the JAK-STAT pathway, facilitating DPV replication and immune evasion in ducks, with Lnc BTU and STAT1 as potential therapeutic targets.
A proposed disease classification system for duck viral hepatitis
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