Book Chapter10.1016/BS.AAMBS.2021.03.003
Lipid production by oleaginous yeasts.
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TL;DR: In this paper, the authors highlight the prominent oleaginous yeast genera, emphasizing their oleagineous characteristics, in conjunction with diverse other features such as cheap carbon source utilization, withstanding the effect of inhibitory compounds, commercially favorable fatty acid composition, and supporting their future development as economically viable lipid feedstock.
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Abstract: Microbial lipid production has been studied extensively for years; however, lipid metabolic engineering in many of the extraordinarily high lipid-accumulating yeasts was impeded by inadequate understanding of the metabolic pathways including regulatory mechanisms defining their oleaginicity and the limited genetic tools available. The aim of this review is to highlight the prominent oleaginous yeast genera, emphasizing their oleaginous characteristics, in conjunction with diverse other features such as cheap carbon source utilization, withstanding the effect of inhibitory compounds, commercially favorable fatty acid composition—all supporting their future development as economically viable lipid feedstock. The unique aspects of metabolism attributing to their oleaginicity are accentuated in the pretext of outlining the various strategies successfully implemented to improve the production of lipid and lipid-derived metabolites. A large number of in silico data generated on the lipid accumulation in certain oleaginous yeasts have been carefully curated, as suggestive evidences in line with the exceptional oleaginicity of these organisms. The different genetic elements developed in these yeasts to execute such strategies have been scrupulously inspected, underlining the major types of newly-found and synthetically constructed promoters, transcription terminators, and selection markers. Additionally, there is a plethora of advanced genetic toolboxes and techniques described, which have been successfully used in oleaginous yeasts in the recent years, promoting homologous recombination, genome editing, DNA assembly, and transformation at remarkable efficiencies. They can accelerate and effectively guide the rational designing of system-wide metabolic engineering approaches pinpointing the key targets for developing industrially suitable yeast strains.
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Citations
Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review
Sameh S. Ali,Rania Al-Tohamy,Tarek Mohamed,Yehia A.-G. Mahmoud,Héctor A. Ruiz,Lushan Sun,Jianzhong Sun +6 more
TL;DR: In this article , the authors used robust oleaginous yeast as a cell factory to convert aromatic wastes to lipids as the substrate for biodiesel transformation, where the highest percentage of lipid accumulation can be achieved as high as 47.25% by Meyerozyma caribbica SSA1654, isolated from a wood-feeding termite gut system.
Combination of simultaneous saccharification and fermentation of corn stover with consolidated bioprocessing of cassava starch enhances lipid production by the amylolytic oleaginous yeast Lipomyces starkeyi.
TL;DR: In this article , a combination of simultaneous saccharification and fermentation of corn stover with consolidated bioprocessing (CBP) of cassava starch by Lipomyces starkeyi was firstly developed as a novel strategy for lipid production.
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Potential of Rhodosporidium toruloides for Fatty Acids Production Using Lignocellulose Biomass.
TL;DR: This review provides useful information on the research working to develop processes for lignocellulose biomass using oleaginous yeast utilizing Rhodosporidium spp.
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Recent advances in genetic technology development of oleaginous yeasts
TL;DR: Recent progress in genetic technology with regard to oleaginous yeasts is reviewed, with a special focus on transformation methods and genome editing tools, discussing the effects of some important genetic parts.
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Production of single cell oil by two novel nonconventional yeast strains of Curvibasidium sp. isolated from medicinal lichen.
TL;DR: In this paper , two cold-adaptive strains of Curvibasidium sp., namely, Y230 and Y231, isolated from the medicinal lichen Usnea diffracta were investigated for their potential in lipid production.
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References
A novel electroporation procedure for highly efficient transformation of Lipomyces starkeyi
Hiroaki Takaku,Atsumi Miyajima,Haruka Kazama,Rikako Sato,Satoshi Ara,Tomohiko Matsuzawa,Katsuro Yaoi,Hideo Araki,Yosuke Shida,Wataru Ogasawara,Harutake Yamazaki +10 more
TL;DR: A novel electroporation transformation method was developed that was first applied for integration of drug-resistance gene markers into the genome of L. starkeyi strain ∆lslig4 at the 18S ribosomal DNA locus of a multiple-copy gene, which yielded approximately 60 transformants/μg of DNA.
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Engineering Candida phangngensis-an oleaginous yeast from the Yarrowia clade-for enhanced detoxification of lignocellulose-derived inhibitors and lipid overproduction.
TL;DR: It is reported that genetic tools already established for use in the latter organism-including promoters, expression vectors, antibiotic resistance genes, a transformation protocol, and the Cre/lox system for marker recycle-can be transferred to the newer member of the Yarrowia clade with little or no need for modifications.
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Evaluation of biodiesels from several oilseed sources as environmental friendly contact herbicides
Steven F. Vaughn,Ronald A Holser +1 more
TL;DR: In this paper, the authors examined the post-emergence contact herbicidal activities of biodiesels from several oilseed sources for potential use to control broadleaf weeds in turfgrasses.
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Production of polyunsaturated fatty acids in recombinant Lipomyces starkeyi through submerged fermentation
TL;DR: This is a first report of DHA enrichment and opens up avenues for LCPUFA production through L. starkeyi, a transformed yeast which can accumulate oil to the extent of 60 % of its biomass and where genetic transformation can be achieved.
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Enhanced microbial lipid production by Cryptococcus albidus in the high-cell-density continuous cultivation with membrane cell recycling and two-stage nutrient limitation
TL;DR: This two-stage continuous cultivation, which provided a higher lipid production performance, shows a great potential for an industrial-scale biotechnological production of microbial lipids and biofuel production.
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